Carcinogenesis, Vol. 21, No. 2, 321-324,
February 2000
© 2000 Oxford University Press
Short Communications |
Oxidative DNA damage in human lymphocytes: correlations with plasma levels of 
-tocopherol and carotenoids
International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France,
1 Department of Community Medicine, Malmö University Hospital, SE-20502 Malmö, Sweden,
2 Escuela Andaluza de Salud Publica, Ap. Correos 2070, E-18080 Granada, Spain and
3 DRFMC/SCIB, Laboratoire des Lésions des Acides Nucléiques, CEA Grenoble, 17 Rue des Martyrs, F38054 Grenoble Cedex 9, France
In order to investigate whether oxidative damage is associated with differences in antioxidant intake, we measured the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in lymphocytes and 
-tocopherol and several carotenoids in plasma of women with different dietary habits. We found that women from Granada (Spain), a region with a typically Mediterranean diet, had significantly higher levels of 8-oxodGuo compared with Malmö (Sweden), a region with a Northern European dietary intake pattern (2.30 ± 0.78 versus 1.59 ± 1.01 8-oxodGuo/10–6 deoxyguanosine). Levels of plasma -tocopherol and carotenoids were higher in Granada and these values were significantly positively correlated with levels of 8-oxodGuo. Our results do not support the hypothesis that a Mediterranean diet rich in -tocopherol and carotenoids protects cells against oxidative DNA damage. It is possible, however, that consumption of foods other than fruits and vegetables, including fats, are responsible for the higher levels of 8-oxodGuo in Granada. Further studies are warranted to better elucidate the role of antioxidants in the modulation of oxidative stress in vivo.
Abbreviations: dG, deoxyguanosine; GC/MS, gas chromatography/mass spectrometry; 5-HMdUrd, 5-hydroxymethyl-2'-deoxyuridine; 8-oxodA, 8-oxo-7,8-dihydro-2'-deoxyadenosine; 8-oxodGuo, 8-oxo-7,8-dihydro-2'-deoxyguanosine.
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