Carcinogenesis, Vol. 21, No. 6, 1117-1120,
June 2000
© 2000 Oxford University Press
Cancer Biology |
Frequent mutations of the ß-catenin gene in mouse colon tumors induced by azoxymethane
Cancer Prevention Division, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan
The ß-catenin gene is frequently mutated at codons 33, 41 and 45 of the glycogen synthase kinase-3ß phosphorylation motif in human colon cancers in patients without APC mutations. Frequent mutations at codons 32 and 34, as well as 33 and 41, have been detected in rat colon tumors induced by azoxymethane (AOM), with the second G of CTGGA sequences being considered as a mutational hot-spot. In the present study, exon 3 of the ß-catenin gene in mouse colon tumors induced by AOM was amplified by PCR and mutations were detected by the single strand conformation polymorphism method, restriction enzyme fragment length polymorphism and direct sequencing. All 10 colon tumors tested were found to have ß-catenin mutations, four in codon 34, three in codon 33, two in codon 41 and one in codon 37, nine being G:C
A:T transitions. However, no mutations were found in codon 32 of the mouse ß-catenin gene. On immmunostaining, ß-catenin was observed in the cytoplasm and nucleus of the tumor cells. The cytoplasmic staining was homogeneous, while both homogeneous and heterogeneous patterns were noted for the nuclei. Highly frequent mutations of the ß-catenin gene in AOM-induced mouse colon tumors suggest that consequent alterations in the stability and localization of the protein may play an important role in this colon carcinogenesis model.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Qiu, E. B. Carson-Walter, S. F. Kuan, L. Zhang, and J. Yu PUMA Suppresses Intestinal Tumorigenesis in Mice Cancer Res., June 15, 2009; 69(12): 4999 - 5006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Fields, S. R. Calcagno, M. Krishna, S. Rak, M. Leitges, and N. R. Murray Protein Kinase C{beta} Is an Effective Target for Chemoprevention of Colon Cancer Cancer Res., February 15, 2009; 69(4): 1643 - 1650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Rosenberg, C. Giardina, and T. Tanaka Mouse models for the study of colon carcinogenesis Carcinogenesis, February 1, 2009; 30(2): 183 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tao, J. Hart, L. Lichtenstein, L. J. Joseph, M. J. Ciancio, S. Hu, E. B. Chang, and M. Bissonnette Inducible heat shock protein 70 prevents multifocal flat dysplastic lesions and invasive tumors in an inflammatory model of colon cancer Carcinogenesis, January 1, 2009; 30(1): 175 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, G. H. McIntosh, R. K. Le Leu, R. Woodman, and G. P. Young Suppression of Colorectal Oncogenesis by Selenium-Enriched Milk Proteins: Apoptosis and K-ras Mutations Cancer Res., June 15, 2008; 68(12): 4936 - 4944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Raufman, R. Samimi, N. Shah, S. Khurana, J. Shant, C. Drachenberg, G. Xie, J. Wess, and K. Cheng Genetic Ablation of M3 Muscarinic Receptors Attenuates Murine Colon Epithelial Cell Proliferation and Neoplasia Cancer Res., May 15, 2008; 68(10): 3573 - 3578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Uronis, H. H. Herfarth, T. C. Rubinas, A. C. Bissahoyo, K. Hanlon, and D. W. Threadgill Flat Colorectal Cancers Are Genetically Determined and Progress to Invasion without Going through a Polypoid Stage Cancer Res., December 15, 2007; 67(24): 11594 - 11600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-C. L. Chang, R. A. Coudry, M. L. Clapper, X. Zhang, K.-L. Williams, C. S. Spittle, T. Li, and H. S. Cooper Loss of p53 enhances the induction of colitis-associated neoplasia by dextran sulfate sodium Carcinogenesis, November 1, 2007; 28(11): 2375 - 2381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Suzuki, H. Kohno, S. Sugie, H. Nakagama, and T. Tanaka Strain differences in the susceptibility to azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice Carcinogenesis, January 1, 2006; 27(1): 162 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Paulsen, E. M. Loberg, H. B. Olstorn, H. Knutsen, I.-L. Steffensen, and J. Alexander Flat Dysplastic Aberrant Crypt Foci Are Related to Tumorigenesis in the Colon of Azoxymethane-Treated Rat Cancer Res., January 1, 2005; 65(1): 121 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tanaka, R. Suzuki, H. Kohno, S. Sugie, M. Takahashi, and K. Wakabayashi Colonic adenocarcinomas rapidly induced by the combined treatment with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate in male ICR mice possess {beta}-catenin gene mutations and increases immunoreactivity for {beta}-catenin, cyclooxygenase-2 and inducible nitric oxide synthase Carcinogenesis, January 1, 2005; 26(1): 229 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dong, K. Guda, P. R. Nambiar, M. Nakanishi, A. C. Lichtler, M. Nishikawa, C. Giardina, and D. W. Rosenberg Azoxymethane-induced pre-adipocyte factor 1 (Pref-1) functions as a differentiation inhibitor in colonic epithelial cells Carcinogenesis, November 1, 2004; 25(11): 2239 - 2246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fujiwara, M. Ochiai, T. Ohta, M. Ohki, H. Aburatani, M. Nagao, T. Sugimura, and H. Nakagama Global gene expression analysis of rat colon cancers induced by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Carcinogenesis, August 1, 2004; 25(8): 1495 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kaneko, K. Morimura, T. Nishikawa, H. Wanibuchi, N. Takada, H. Osugi, H. Kinoshita, and S. Fukushima Different genetic alterations in rat forestomach tumors induced by genotoxic and non-genotoxic carcinogens Carcinogenesis, October 1, 2002; 23(10): 1729 - 1735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Gupta and R. N. Dubois Controversy: PPARgamma as a target for treatment of colorectal cancer Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G266 - G269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takagi, Y. Nakano, S. Takekoshi, T. Inagami, and M. Tamura Hemizygous mice for the angiotensin II type 2 receptor gene have attenuated susceptibility to azoxymethane-induced colon tumorigenesis Carcinogenesis, July 1, 2002; 23(7): 1235 - 1241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Koesters, M. A. Hans, A. Benner, R. Prosst, J. Boehm, J. Gahlen, and M. von Knebel Doeberitz Predominant mutation of codon 41 of the {beta}-catenin proto-oncogene in rat colon tumors induced by 1,2-dimethylhydrazine using a complete carcinogenic protocol Carcinogenesis, November 1, 2001; 22(11): 1885 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Erik Paulsen, I.-L. Steffensen, E. M. Loberg, T. Husoy, E. Namork, and J. Alexander Qualitative and Quantitative Relationship between Dysplastic Aberrant Crypt Foci and Tumorigenesis in the Min/+ Mouse Colon Cancer Res., July 1, 2001; 61(13): 5010 - 5015. [Abstract] [Full Text] [PDF]
|
|