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Carcinogenesis, Vol. 21, No. 6, 1121-1127, June 2000
© 2000 Oxford University Press

Cancer Biology

Biologic instability of pancreatic cancer xenografts in the nude mouse

Bruno M. Schmied1,2, Alexis B. Ulrich1,3, Hosei Matsuzaki1,4, Tarek H. El-Metwally5, Xianzhong Ding5, Mirabella E. Fernandes6, Thomas E. Adrian5, William G. Chaney6, Surinder K. Batra6 and Parviz M. Pour1,7,8

1 UNMC Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA,
2 Department of Visceral and Transplantation Surgery, Insel Hospital, Bern, Switzerland,
3 Department of Surgery, Rheinische Friedrich-Wilhelms-University, Bonn, Germany,
4 Department of Surgery II, Kumamoto University, Kumamoto, Japan,
5 Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE,
6 Department of Biochemistry and Molecular Biology and
7 Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA

Tumor transplants into nude mice (NM) may reveal abnormal biological behavior compared with the original tumor. Despite this, human tumor xenografts in NM have been widely used to study the biology of tumors and to establish diagnostic and therapeutic modalities. Clearly, precise differences in the biology of a given tumor in human and in NM cannot be assessed. We compared the growth kinetics, differentiation pattern and karyotype of an anaplastic Syrian hamster pancreatic cancer cell line in NM and in allogenic hamsters. As with the original tumor, transplants in hamsters grew fast, were anaplastic and expressed markers related to tumor malignancy like galectin 3, TGF-{alpha} and its receptor EGFR at high levels. However, tumors in the NM were well-differentiated adenocarcinomas, grew slower, had increased apoptotic rate and had a high expression of differentiation markers such as blood group A antigen, DU-PAN-2, carbonic anhydrase II, TGF-ß2 and mucin. Karyotypically, the tumors in the NM acquired additional chromosomal damage. Our results demonstrate significant differences in the morphology and biology of tumors grown in NM and the allogenic host, and call for caution in extrapolating data obtained from xenografts to primary cancer.


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