Carcinogenesis, Vol. 21, No. 8, 1581-1586,
August 2000
© 2000 Oxford University Press
Carcinogenesis |
Multicolour FISH detection of radioactive iodine-induced 17cen–p53 chromosomal breakage in buccal cells from therapeutically exposed patients
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra,
1 Servei de Medicina Nuclear, Ciutat Sanitària i Universitària Vall d'Hebron, Pg. Vall d'Hebron 119, 08035 Barcelona, Spain and
2 School of Biological Sciences, University of Wales, Swansea, Singleton Park, Swansea, UK
Simultaneous labelling of 17cen and the p53 locus by multicolour FISH was used to monitor radioactive iodine-induced structural and numerical chromosome abnormalities in buccal cells from 29 hyperthyroidism and thyroid cancer patients sampled before and after therapeutic treatment. This novel methodology allowed the efficient detection of 17p deletions leading to p53 allelic deletions, 17p gains and whole chromosome 17 numerical abnormalities in epithelial cells. Highly significant increases in the frequency of cells with (i) 17p abnormalities (1.8-fold; P < 0.001), including p53 monoallelic deletions (2.1-fold; P < 0.001) and 17p gains (3.5-fold; P < 0.001); (ii) chromosome 17 numerical abnormalities (2-fold; P < 0.001); and (iii) simultaneous 17p breakage and chromosome 17 numerical abnormalities (2.3-fold; P < 0.001), were observed after radioactive iodine treatment. As expected, the major contribution to these increases was detected in hyperthyroidism patients compared with thyroid cancer patients who suffered thyroidectomy before radioactive iodine exposure and, therefore, experienced a rapid elimination of the radioisotope. Considering that both the genetic endpoints and the target tissue are extremely relevant in carcinogenesis, it is suggested that the observed genetic damage could contribute to the reported increase in cancer risk of people therapeutically or accidentally exposed to radioactive iodine.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L Popova, D Kishkilova, V. Hadjidekova, R. Hristova, P Atanasova, V. Hadjidekova, D Ziya, and V. Hadjidekov Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography Dentomaxillofac. Radiol., March 1, 2007; 36(3): 168 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Thomas and M. Fenech A review of genome mutation and Alzheimer's disease Mutagenesis, January 1, 2007; 22(1): 15 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Puerto, M.J. Ramirez, R. Marcos, A. Creus, and J. Surralles Radiation-induced chromosome aberrations in human euchromatic (17cen-p53) and heterochromatic (1cen-1q12) regions Mutagenesis, July 1, 2001; 16(4): 291 - 296. [Abstract] [Full Text] [PDF]
|
|