Carcinogenesis, Vol. 22, No. 1, 187-191,
January 2001
© 2001 Oxford University Press
SHORT COMMUNICATION |
Expression of 15-lipoxygenase-1 is regulated by histone acetylation in human colorectal carcinoma
1 Laboratory of Molecular Carcinogenesis, National Institutes of Environmental Health Sciences, Research Triangle Park, NC 27709, USA,
2 Division of Neurosurgery, Institute of Neurological Sciences, Tottori University School of Medicine, 36-1 Nishi-cho, Yonago 683-0805, Japan and
3 Renal Division and Glomerulonephritis Center, Emory University, Atlanta, GA 30322, USA
15-Lipoxygenase-1 (15-LO-1) is expressed at higher levels in human colorectal tumors compared with normal tissue. 15-LO-1 is expressed in cultured human colorectal cells, but only after treatment with sodium butyrate (NaBT), which also stimulates apoptosis and cell differentiation. We examined the regulation of 15-LO-1 in human tissue and the colorectal carcinoma cell lines Caco-2 and SW-480 by treatment with histone deacetylase (HDAC) inhibitors: NaBT, trichostatin A (TSA) and HC toxin. Northern and western analysis showed that expression of 15-LO-1 was up-regulated by these HDAC inhibitors. Furthermore, HDAC inhibitors stimulated promoter activity of the 15-LO-1 gene ~12-to 21-fold using the –331/–23 region of the 15-LO-1 promoter, as measured with a luciferase–15-LO-1 promoter–reporter system, suggesting that 15-LO-1 is regulated at the transcriptional level by HDAC inhibitors. Histone proteins in colorectal cells were acetylated after treatment with HDAC inhibitors. Histone acetylation was also measured in human colorectal tissue and a correlation was observed between increased histone acetylation and 15-LO-1 expression. Thus, regulation of 15-LO-1 expression in colorectal tissues appears to occur by a novel and new mechanism associated with histone acetylation. Moreover, these results suggest that 15-LO-1 is a marker that reflects histone acetylation in colorectal carcinoma.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Zuo, J. S. Morris, and I. Shureiqi Chromatin Modification Requirements for 15-Lipoxygenase-1 Transcriptional Reactivation in Colon Cancer Cells J. Biol. Chem., November 14, 2008; 283(46): 31341 - 31347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zuo, L. Shen, J.-P. Issa, O. Moy, J. S. Morris, S. M. Lippman, and I. Shureiqi 15-Lipoxygenase-1 transcriptional silencing by DNA methyltransferase-1 independently of DNA methylation FASEB J, June 1, 2008; 22(6): 1981 - 1992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Shureiqi, X. Zuo, R. Broaddus, Y. Wu, B. Guan, J. S. Morris, and S. M. Lippman The transcription factor GATA-6 is overexpressed in vivo and contributes to silencing 15-LOX-1 in vitro in human colon cancer FASEB J, March 1, 2007; 21(3): 743 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Deguchi, S. W. Xing, I. Shureiqi, P. Yang, R. A. Newman, S. M. Lippman, S. J. Feinmark, B. Oehlen, and I. B. Weinstein Activation of Protein Kinase G Up-regulates Expression of 15-Lipoxygenase-1 in Human Colon Cancer Cells Cancer Res., September 15, 2005; 65(18): 8442 - 8447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Kim, S. J. Baek, F. G. Bottone Jr., T. Sali, and T. E. Eling Overexpression of 15-Lipoxygenase-1 Induces Growth Arrest through Phosphorylation of p53 in Human Colorectal Cancer Cells Mol. Cancer Res., September 1, 2005; 3(9): 511 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tang, B. Bhatia, C. J. Maldonado, P. Yang, R. A. Newman, J. Liu, D. Chandra, J. Traag, R. D. Klein, S. M. Fischer, et al. Evidence That Arachidonate 15-Lipoxygenase 2 Is a Negative Cell Cycle Regulator in Normal Prostate Epithelial Cells J. Biol. Chem., May 3, 2002; 277(18): 16189 - 16201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Taniura, H. Kamitani, T. Watanabe, and T. E. Eling Transcriptional Regulation of Cyclooxygenase-1 by Histone Deacetylase Inhibitors in Normal Human Astrocyte Cells J. Biol. Chem., May 3, 2002; 277(19): 16823 - 16830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tsubaki, V. Hwa, S. M. Twigg, and R. G. Rosenfeld Differential Activation of the IGF Binding Protein-3 Promoter by Butyrate in Prostate Cancer Cells Endocrinology, May 1, 2002; 143(5): 1778 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Uhl, N. Klan, M. Rose, K.-D. Entian, O. Werz, and D. Steinhilber The 5-Lipoxygenase Promoter Is Regulated by DNA Methylation J. Biol. Chem., February 1, 2002; 277(6): 4374 - 4379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Shankaranarayanan, P. Chaitidis, H. Kuhn, and S. Nigam Acetylation by Histone Acetyltransferase CREB-binding Protein/p300 of STAT6 Is Required for Transcriptional Activation of the 15-Lipoxygenase-1 Gene J. Biol. Chem., November 9, 2001; 276(46): 42753 - 42760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Hsi, L. Wilson, J. Nixon, and T. E. Eling 15-Lipoxygenase-1 Metabolites Down-regulate Peroxisome Proliferator-activated Receptor gamma via the MAPK Signaling Pathway J. Biol. Chem., September 7, 2001; 276(37): 34545 - 34552. [Abstract] [Full Text] [PDF]
|
|