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Carcinogenesis, Vol. 22, No. 2, 215-219, February 2001
© 2001 Oxford University Press

CANCER BIOLOGY

NBP is the p53 homolog p63

Xiaoya Zeng, Yong Zhu and Hua Lu,

Department of Biochemistry and Molecular Biology, Oregon Health Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA

We previously identified a non-p53, p53-responsive DNA element (p53RE)-binding protein named NBP, functionally analogous to p53, from human cervical carcinoma Hela cells. Here we report a biochemical study demonstrating that this activity is the recently cloned p53 analog p63. NBP was purified through conventional and DNA affinity chromatography to apparent homogeneity with a prominent polypeptide migrating in between the 43 and 68 kDa positions on a SDS gel. This polypeptide immunoreacted with monoclonal anti-p63 but not anti-p53 or anti-p73 antibodies. Also, NBP co-purified with p63 through each step of fractionation, as detected with anti-p63 antibodies. DNA–protein complexes formed with purified NBP and p53RE-containing oligomers derived from the p21waf1 promoter were supershifted by anti-p63 but not anti-p53 antibodies. Thus, these results demonstrate that NBP is encoded by the p53 homolog p63 gene.


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