Carcinogenesis, Vol. 22, No. 9, 1355-1362,
September 2001
© 2001 Oxford University Press
CANCER BIOLOGY |
OGG1 protein suppresses G:C
T:A mutation in a shuttle vector containing 8-hydroxyguanine in human cells
1 Biology Division, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045,
2 First Department of Internal Medicine, Gunma University School of Medicine, 39-15 Showa-machi 3-chome, Gunma 371-8511 and
3 Research Center for Environmental Quality Control, Kyoto University, 1-2 Yumihara, Otsu, Shiga 520, Japan
8-Hydroxyguanine (8-OHG) is an oxidatively damaged mutagenic base which causes G:C
T:A transversions in DNA. OGG1 was cloned as a human gene encoding a DNA glycosylase that specifically excises 8-OHG from DNA in vitro. However, it was not clear whether OGG1 protein suppresses G:CT:A transversions caused by 8-OHG in human cells in vivo. In the present study we have examined the ability of OGG1 protein to suppress G:CT:A transversions caused by 8-OHG in human cells by bacterial suppressor tRNA (supF) forward mutation assay using a shuttle vector DNA, pMY189. Introduction of a single 8-OHG residue at position 159 of the supF gene in plasmid pMY189 resulted in a 130-fold increase in mutation frequency compared with untreated plasmid pMY189 after replication in the NCI-H1299 human lung cancer cell line. G:CT:A transversions at position 159 were detected in >90% of the supF mutants from the 8-OHG-containing plasmid. The mutation frequency of the 8-OHG-containing plasmid was significantly reduced by overexpression of OGG1 protein in NCI-H1299 cells and, in particular, the occurrence of G:CT:A transversion at position 159 in the supF gene was suppressed. Furthermore, frequencies and spectra of mutations of the untreated pMY189 plasmid did not differ significantly with overexpression of OGG1 protein. These results indicate that OGG1 protein has the ability to suppress G:CT:A transversions caused by 8-OHG in human cells in vivo.
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