Carcinogenesis, Vol. 23, No. 1, 213-216,
January 2002
© 2002 Oxford University Press
SHORT COMMUNICATION |
Increased frequency of DNA deletions in pink-eyed unstable mice carrying a mutation in the Werner syndrome gene homologue
Centre de Recherche en Cancérologie de l'Université Laval, Pavillon Hôtel-Dieu de Québec, CHUQ, 9 McMahon Street, Québec City, Québec G1R 2J6, Canada
Werner syndrome (WS) is a rare autosomal recessive disorder characterized by genomic instability and the premature onset of a number of age-related diseases, including cancers. Accumulating evidence indicates that the WS gene product is involved in resolving aberrant DNA structures that may arise during the process of DNA replication and/or transcription. To estimate the frequency of DNA deletions directly in the skin of mouse embryos, mice with a deletion of part of the murine WRN helicase domain were created. These mutant mice were then crossed to the pink-eyed unstable animals, which have a 70 kb internal duplication at the pink-eyed dilution (p) gene. This report indicates that the frequency of deletion of the duplicated sequence at the p locus is elevated in mice with a mutation in the WRN allele when compared with wild-type mice. In addition, the inhibitor of topoisomerase I camptothecin also increases the frequency of deletion at the p locus. This frequency is even more elevated in WRN mutant mice treated with camptothecin. In contrast, while the inhibition of poly(ADP-ribose) polymerase (PARP) activity by 3-aminobenzamide increases the frequency of DNA deletion, mutant WRN mice are not significantly more sensitive to the inhibition of PARP activity than wild-type animals.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. W. Ku, J. Aubrecht, R. J. Mauthe, R. H. Schiestl, and A. J. Fornace Jr Genetic Toxicity Assessment: Employing the Best Science for Human Safety Evaluation Part VII: Why Not Start with a Single Test: A Transformational Alternative to Genotoxicity Hazard and Risk Assessment Toxicol. Sci., September 1, 2007; 99(1): 20 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Kirpnick-Sobol, R. Reliene, and R. H. Schiestl Carcinogenic Cr(VI) and the Nutritional Supplement Cr(III) Induce DNA Deletions in Yeast and Mice. Cancer Res., April 1, 2006; 66(7): 3480 - 3484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Reliene and R. H. Schiestl Glutathione depletion by buthionine sulfoximine induces DNA deletions in mice Carcinogenesis, February 1, 2006; 27(2): 240 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Deschenes, L. Massip, C. Garand, and M. Lebel In vivo misregulation of genes involved in apoptosis, development and oxidative stress in mice lacking both functional Werner syndrome protein and poly(ADP-ribose) polymerase-1 Hum. Mol. Genet., November 1, 2005; 14(21): 3293 - 3308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Leder, J. McMenamin, K. Fontaine, A. Bishop, and P. Leder {zeta}-/- Thalassemic mice are affected by two modifying loci and display unanticipated somatic recombination leading to inherited variation Hum. Mol. Genet., March 1, 2005; 14(5): 615 - 625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Kovalchuk, C. A. Hendricks, S. Cassie, A. J. Engelward, and B. P. Engelward In vivo Recombination After Chronic Damage Exposure Falls to Below Spontaneous Levels in "Recombomice" Mol. Cancer Res., October 1, 2004; 2(10): 567 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Reliene, E. Fischer, and R. H. Schiestl Effect of N-Acetyl Cysteine on Oxidative DNA Damage and the Frequency of DNA Deletions in Atm-Deficient Mice Cancer Res., August 1, 2004; 64(15): 5148 - 5153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. R. Bishop, M. C. Hollander, B. Kosaras, R. L. Sidman, A. J. Fornace Jr., and R. H. Schiestl Atm-, p53-, and Gadd45a-Deficient Mice Show an Increased Frequency of Homologous Recombination at Different Stages during Development Cancer Res., September 1, 2003; 63(17): 5335 - 5343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Hendricks, K. H. Almeida, M. S. Stitt, V. S. Jonnalagadda, R. E. Rugo, G. F. Kerrison, and B. P. Engelward Spontaneous mitotic homologous recombination at an enhanced yellow fluorescent protein (EYFP) cDNA direct repeat in transgenic mice PNAS, May 27, 2003; 100(11): 6325 - 6330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lebel, J. Lavoie, I. Gaudreault, M. Bronsard, and R. Drouin Genetic Cooperation between the Werner Syndrome Protein and Poly(ADP-Ribose) Polymerase-1 in Preventing Chromatid Breaks, Complex Chromosomal Rearrangements, and Cancer in Mice Am. J. Pathol., May 1, 2003; 162(5): 1559 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Stephan, F. Grosse, and K. Soe Human topoisomerase I cleavage complexes are repaired by a p53-stimulated recombination-like reaction in vitro Nucleic Acids Res., December 1, 2002; 30(23): 5087 - 5093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Werner mouse Wrn-{Delta}hel Sci. Aging Knowl. Environ., April 3, 2002; 2002(13): tg4 - 4. [Full Text]
|
|