Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Potter, A. J.
Right arrow Articles by Rabinovitch, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Potter, A. J.
Right arrow Articles by Rabinovitch, P. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol. 23, No. 3, 389-401, March 2002
© 2002 Oxford University Press

CANCER BIOLOGY

Flow cytometric analysis of the cell cycle phase specificity of DNA damage induced by radiation, hydrogen peroxide and doxorubicin

Alan J. Potter1, Katherine A. Gollahon1, Ben J.A. Palanca1,3, Mary J. Harbert1, Young M. Choi1,4, Alexander H. Moskovitz1,5, John D. Potter2 and Peter S. Rabinovitch1,2,6

1 Department of Pathology, University of Washington, Seattle, WA 98195,
2 Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA

We have optimized a flow cytometric DNA alkaline unwinding assay to increase the sensitivity in detecting low levels of DNA damage (strand breaks and alkali-labile sites) and to permit the measurement of the extent of DNA damage within each cell cycle compartment. The lowest {gamma} radiation dose that induced detectable DNA damage in each cell cycle phase of HeLa and CEM cells was 10 cGy. The lowest H2O2 concentration that induced detectable DNA damage in each cell cycle phase was 0.5 µM in HeLa cells, and 1–2.5 TµM in CEM cells. For both HeLa cells and CEM cells, DNA damage in each cell cycle compartment increased approximately linearly with increasing doses of {gamma} radiation and H2O2. Although untreated HeLa and CEM cells in S phase consistently exhibited greater DNA unwinding than did G1 or G2 cells (presumably due to DNA strand breaks associated with replication forks), there was no difference between the susceptibility of G0/G1, S and G2/M phase cells to DNA damage induced by {gamma} radiation or H2O2, or in the rate of repair of this damage. In each cell cycle phase, the susceptibility to {gamma} radiation-induced DNA damage was greater in CEM cells than in HeLa cells. In contrast to the lack of cell cycle phase-specific DNA damage induced by exposure to {gamma} radiation or H2O2, the cancer chemotherapeutic drug doxorubicin (adriamycin) predominantly induced DNA damage in G2 phase cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
P. Ubezio, M. Lupi, D. Branduardi, P. Cappella, E. Cavallini, V. Colombo, G. Matera, C. Natoli, D. Tomasoni, and M. D'Incalci
Quantitative Assessment of the Complex Dynamics of G1, S, and G2-M Checkpoint Activities
Cancer Res., June 15, 2009; 69(12): 5234 - 5240.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
B. Thyagarajan, K. E. Anderson, C. J. Lessard, G. Veltri, D. R. Jacobs, A. R. Folsom, and M. D. Gross
Alkaline unwinding flow cytometry assay to measure nucleotide excision repair
Mutagenesis, March 1, 2007; 22(2): 147 - 153.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
B. Hutter, C. Schaab, S. Albrecht, M. Borgmann, N. A. Brunner, C. Freiberg, K. Ziegelbauer, C. O. Rock, I. Ivanov, and H. Loferer
Prediction of Mechanisms of Action of Antibacterial Compounds by Gene Expression Profiling
Antimicrob. Agents Chemother., August 1, 2004; 48(8): 2838 - 2844.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J.-H. Yuan, Y. Feng, R. H. Fisher, S. Maloid, D. L. Longo, and D. K. Ferris
Polo-Like Kinase 1 Inactivation Following Mitotic DNA Damaging Treatments Is Independent of Ataxia Telangiectasia Mutated Kinase
Mol. Cancer Res., July 1, 2004; 2(7): 417 - 426.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. S. DiPaola
To Arrest or Not To G2-M Cell-Cycle Arrest : Commentary re: A. K. Tyagi et al., Silibinin Strongly Synergizes Human Prostate Carcinoma DU145 Cells to Doxorubicin-induced Growth Inhibition, G2-M Arrest, and Apoptosis. Clin. Cancer Res., 8: 3512-3519, 2002.
Clin. Cancer Res., November 1, 2002; 8(11): 3311 - 3314.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.