Carcinogenesis, Vol. 23, No. 9, 1483-1489,
September 2002
© 2002 Oxford University Press
MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION |
Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean population
1 Department of Pharmacology, University of Florence,
2 Molecular and Nutritional Epidemiology Unit, Centro per lo Studio e la Prevenzione Oncologica (CSPO), Istituto Scientifico della Regione Toscana, Florence, Italy and
3 Nutrition and Cancer Unit, IARC, Lyon, France
In order to evaluate dietary and lifestyle determinants of oxidative DNA damage we used a modification of the ‘comet assay’ (single cell alkaline gel electrophoresis), with the fpg enzyme (formamidopyrimidine DNA glycosilase), to measure the basal level of DNA oxidation in peripheral lymphocytes donated by 71 healthy adults living in Florence, Italy. Detailed information about dietary and lifestyle habits was collected by two validated and standardized questionnaires; we also measured plasma concentrations of selected micro-nutrients (six carotenoids, retinol, 
- and 
-tocopherol). DNA damage, measured as percent DNA migrated in the comet tail (mean 4.67%, interquartile range 2.36–6.62%), was not associated with gender, age, weight, body mass index, physical activity or smoking history. A positive correlation with height and period of blood sampling emerged: DNA damage tended to be higher among taller subjects (P = 0.02) and in samples obtained in summer months (P = 0.02). Multivariate analyses showed a positive association with coffee (P = 0.01) and tomato consumption (P = 0.05). Instead, the consumption of cruciferous vegetables tended to be negatively associated with oxidative damage (P = 0.09). Furthermore, a positive non-significant association between the consumption of total vegetables and fresh fruit and DNA damage emerged (P = 0.08 and P = 0.10, respectively). The estimated intake of simple sugars showed a strong positive association with oxidative DNA damage (P = 0.01), while vitamin E showed a borderline positive association (P = 0.06). The plasma levels of several micro-nutrients did not appear to influence DNA damage. Our results, although based on a relatively small group of subjects, indicate that individual dietary and lifestyle habits only modestly affect the levels of lymphocyte DNA oxidation and suggest that specific dietary patterns, rich in fresh fruit and vegetables, are not clearly related to decreased oxidative damage in peripheral lymphocytes in a Mediterranean population.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Battershill, K. Burnett, and S. Bull Factors affecting the incidence of genotoxicity biomarkers in peripheral blood lymphocytes: impact on design of biomonitoring studies Mutagenesis, August 4, 2008; (2008) gen040v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu and K. Morimoto Exposure level to cigarette tar or nicotine is associated with leukocyte DNA damage in male Japanese smokers Mutagenesis, June 27, 2008; (2008) gen034v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dusinska and A. R. Collins The comet assay in human biomonitoring: gene-environment interactions Mutagenesis, May 1, 2008; 23(3): 191 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Thomson, N. R. Stendell-Hollis, C. L. Rock, E. C. Cussler, S. W. Flatt, and J. P. Pierce Plasma and Dietary Carotenoids Are Associated with Reduced Oxidative Stress in Women Previously Treated for Breast Cancer Cancer Epidemiol. Biomarkers Prev., October 1, 2007; 16(10): 2008 - 2015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Watters, J. A. Satia, L. L. Kupper, J. A. Swenberg, J. C. Schroeder, and B. R. Switzer Associations of Antioxidant Nutrients and Oxidative DNA Damage in Healthy African-American and White Adults Cancer Epidemiol. Biomarkers Prev., July 1, 2007; 16(7): 1428 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hoffmann, J. Hogel, and G. Speit The effect of smoking on DNA effects in the comet assay: a meta-analysis Mutagenesis, November 1, 2005; 20(6): 455 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lee, N. J. Hodges, and J. K. Chipman Interindividual Variability in Response to Sodium Dichromate-Induced Oxidative DNA Damage: Role of the Ser326Cys Polymorphism in the DNA-Repair Protein of 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine DNA Glycosylase 1 Cancer Epidemiol. Biomarkers Prev., February 1, 2005; 14(2): 497 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I.R. Gill, S. Haldar, S. Porter, S. Matthews, S. Sullivan, J. Coulter, H. McGlynn, and I. Rowland The Effect of Cruciferous and Leguminous Sprouts on Genotoxicity, In vitro and In vivo Cancer Epidemiol. Biomarkers Prev., July 1, 2004; 13(7): 1199 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Palli, G. Masala, P. Vineis, S. Garte, C. Saieva, V. Krogh, S. Panico, R. Tumino, A. Munnia, E. Riboli, et al. Biomarkers of dietary intake of micronutrients modulate DNA adduct levels in healthy adults Carcinogenesis, April 1, 2003; 24(4): 739 - 746. [Abstract] [Full Text] [PDF]
|
|