Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Boccellino, M.
Right arrow Articles by Quagliuolo, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boccellino, M.
Right arrow Articles by Quagliuolo, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol. 24, No. 3, 535-540, March 2003
© 2003 Oxford University Press

CARCINOGENESIS

Styrene-7,8-oxide activates a complex apoptotic response in neuronal PC12 cell line

Mariarosaria Boccellino1, Franca Cuccovillo2, Maria Napolitano3, Nicola Sannolo4, Ciro Balestrieri1, Antonio Acampora2, Alfonso Giovane1 and Lucio Quagliuolo1

1 Dipartimento di Biochimica e Biofisica, Seconda Università degli Studi di Napoli, Via Costantinopoli 16, I-80138 Napoli, Italy
2 Dipartimento di Medicina Pubblica e Sicurezza Sociale, Università degli Studi di Napoli ‘Federico II’, Via S. Pansini 5, I-80131 Napoli, Italy
3 Istituto Nazionale per la Cura e lo Studio dei Tumori, Fondazione ‘Sen. G. Pascale’, Via M. Semmola, I-80131 Napoli, Italy
4 Dipartimento di Medicina Sperimentale, Sezione di Medicina del Lavoro, Igiene e Tossicologia Professionale, Seconda Università degli Studi di Napoli, Piazza Miraglia 2, I-80134 Napoli, Italy

Styrene-7,8-oxide (SO), the major in vivo metabolite of styrene, one of the most important plastic monomers worldwide, is classified as carcinogenic in humans and animals. Although the toxic effects of SO have been extensively documented in human lymphocytes, the molecular mechanisms responsible for SO-induced cell damage are still unknown. In the present study, we evaluated the effect of SO on growth and apoptosis, assessed by FACS and gel ladder analysis, in neuronal PC12 cell line. Our results demonstrate that SO triggered PC12 cell apoptosis in a dose- and time-dependent manner. PC12 apoptosis was associated with caspase-3 activation and modulation of the Bcl-2 family proteins. In addition, examination of the cytoskeleton showed that SO induced F-actin depolymerization and a rapid cell rounding before caspase-3 activation, suggesting that the changes in cell shape involving cytoskeletal structure are an early step in the apoptotic pathway. Therefore, SO triggers a complex apoptotic response consisting of a loss of cytoskeletal organization that precedes caspase-3 activation. These mechanisms may represent the molecular basis of the different SO sensitivity to tumor promotion among species and organs.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
G.-D. Chen, L.-H. Chi, P. J. Kostyniak, and D. Henderson
Styrene Induced Alterations in Biomarkers of Exposure and Effects in the Cochlea: Mechanisms of Hearing Loss
Toxicol. Sci., July 1, 2007; 98(1): 167 - 177.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
R. Cozzolino, P. Palladino, F. Rossi, G. Cali, E. Benedetti, and P. Laccetti
Antineoplastic cyclic astin analogues kill tumour cells via caspase-mediated induction of apoptosis
Carcinogenesis, April 1, 2005; 26(4): 733 - 739.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.