Skip Navigation


Carcinogenesis Advance Access originally published online on May 19, 2005
Carcinogenesis 2005 26(10):1731-1740; doi:10.1093/carcin/bgi134
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
26/10/1731    most recent
bgi134v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yang, Y.-G.
Right arrow Articles by Wang, Z.-Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.-G.
Right arrow Articles by Wang, Z.-Q.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis vol.26 no.10 © Oxford University Press 2005; all rights reserved.

The Fanconi anemia group A protein modulates homologous repair of DNA double-strand breaks in mammalian cells

Yun-Gui Yang, Zdenko Herceg, Koji Nakanishi 1, Ilja Demuth 2, Colette Piccoli, Jocelyne Michelon, Gabriele Hildebrand 2, Maria Jasin 1, Martin Digweed 2 and Zhao-Qi Wang *

International Agency for Research on Cancer (IARC), 150 cours Albert Thomas, F-69008 Lyon, France, 1 Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA and 2 Institut fur Humangenetik, Charite-Campus Virchow-Klinikum, Humboldt Universitat zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany

* To whom correspondence should be addressed. Tel: +33 4 72 73 85 10; Fax:+33 4 72 73 83 29; Email: zqwang{at}iarc.fr

Fanconi anemia (FA) cells exhibit hypersensitivity to DNA interstrand cross-links (ICLs) and high levels of chromosome instability. FA gene products have been shown to functionally or physically interact with BRCA1, RAD51 and the MRE11/RAD50/NBS1 complex, suggesting that the FA complex may be involved in the repair of DNA double-strand breaks (DSBs). Here, we have investigated specifically the function of the FA group A protein (FANCA) in the repair of DSBs in mammalian cells. We show that the targeted deletion of Fanca exons 37–39 generates a null for Fanca in mice and abolishes ubiquitination of Fancd2, the downstream effector of the FA complex. Cells lacking Fanca exhibit increased chromosomal aberrations and attenuated accumulation of Brca1 and Rad51 foci in response to DNA damage. The absence of Fanca greatly reduces gene-targeting efficiency in mouse embryonic stem (ES) cells and compromises the survival of fibroblast cells in response to ICL agent treatment. Fanca-null cells exhibit compromised homology-directed repair (HDR) of DSBs, particularly affecting the single-strand annealing pathway. These data identify the Fanca protein as an integral component in the early step of HDR of DSBs and thereby minimizing the genomic instability.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. Kitao, K. Yamamoto, N. Matsushita, M. Ohzeki, M. Ishiai, and M. Takata
Functional Interplay between BRCA2/FancD1 and FancC in DNA Repair
J. Biol. Chem., July 28, 2006; 281(30): 21312 - 21320.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. Zhu and A. Dutta
An ATR- and BRCA1-Mediated Fanconi Anemia Pathway Is Required for Activating the G2/M Checkpoint and DNA Damage Repair upon Rereplication
Mol. Cell. Biol., June 15, 2006; 26(12): 4601 - 4611.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Taniguchi and A. D. D'Andrea
Molecular pathogenesis of Fanconi anemia: recent progress
Blood, June 1, 2006; 107(11): 4223 - 4233.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. D. Kennedy and A. D. D'Andrea
The Fanconi Anemia/BRCA pathway: new faces in the crowd
Genes & Dev., December 15, 2005; 19(24): 2925 - 2940.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.