Carcinogenesis Advance Access originally published online on October 28, 2004
Carcinogenesis 2005 26(2):495-502; doi:10.1093/carcin/bgh321
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Carcinogenesis vol.26 no.2 © Oxford University Press 2005; all rights reserved.
ARTICLE |
Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis
1 National Food Safety Toxicology Center, Department of Pediatrics and Human Development and 2 Department of Physiology, Michigan State University, East Lansing, MI 48824, USA
* To whom correspondence should be addressed Tel: +517 432 3100; Fax: +1 517 432 6340; Email: james.trosko{at}ht.msu.edu
The Oct3/4 gene, a POU family transcription factor, has been noted as being specifically expressed in embryonic stem cells and in tumor cells but not in cells of differentiated tissues. With the ability to isolate adult human stem cells it became possible to test for the expression of Oct3/4 gene in adult stem cells and to test the stem cell theory of carcinogenesis. Using antibodies and PCR primers we tested human breast, liver, pancreas, kidney, mesenchyme and gastric stem cells, the cancer cell lines HeLa and MCF-7 and human, dog and rat tumors for Oct4 expression. The results indicate that adult human stem cells, immortalized non-tumorigenic cells and tumor cells and cell lines, but not differentiated cells, express Oct4. Oct4 is expressed in a few cells found in the basal layer of human skin epidermis. The data demonstrate that adult stem cells maintain expression of Oct4, consistent with the stem cell hypothesis of carcinogenesis.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Kim, E. Dere, L. D. Burgoon, C.-C. Chang, and T. R. Zacharewski Comparative Analysis of AhR-Mediated TCDD-Elicited Gene Expression in Human Liver Adult Stem Cells Toxicol. Sci., November 1, 2009; 112(1): 229 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. L. Muehlberg, Y.-H. Song, A. Krohn, S. P. Pinilla, L. H. Droll, X. Leng, M. Seidensticker, J. Ricke, A. M. Altman, E. Devarajan, et al. Tissue-resident stem cells promote breast cancer growth and metastasis Carcinogenesis, April 1, 2009; 30(4): 589 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Trosko REVIEW PAPER: Cancer Stem Cells and Cancer Nonstem Cells: From Adult Stem Cells or from Reprogramming of Differentiated Somatic Cells Vet. Pathol., March 1, 2009; 46(2): 176 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Ting, B. F. Kimler, C. J. Fabian, and B. K. Petroff Tamoxifen Prevents Premalignant Changes of Breast, but not Ovarian, Cancer in Rats at High Risk for Both Diseases Cancer Prevention Research, December 1, 2008; 1(7): 546 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hu, S. Liu, D. R. Breiter, F. Wang, Y. Tang, and S. Sun Octamer 4 Small Interfering RNA Results in Cancer Stem Cell-Like Cell Apoptosis Cancer Res., August 15, 2008; 68(16): 6533 - 6540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Chang, G.-S. Shieh, P. Wu, C.-C. Lin, A.-L. Shiau, and C.-L. Wu Oct-3/4 Expression Reflects Tumor Progression and Regulates Motility of Bladder Cancer Cells Cancer Res., August 1, 2008; 68(15): 6281 - 6291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Monk, M. Hitchins, and S. Hawes Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development Mol. Hum. Reprod., June 1, 2008; 14(6): 347 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Quinlan, S. E. Quatela, M. R. Philips, and J. Settleman Activated Kras, but Not Hras or Nras, May Initiate Tumors of Endodermal Origin via Stem Cell Expansion Mol. Cell. Biol., April 15, 2008; 28(8): 2659 - 2674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tang, K. Kitisin, W. Jogunoori, C. Li, C.-X. Deng, S. C. Mueller, H. W. Ressom, A. Rashid, A. R. He, J. S. Mendelson, et al. Progenitor/stem cells give rise to liver cancer due to aberrant TGF-{beta} and IL-6 signaling PNAS, February 19, 2008; 105(7): 2445 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-L. Wu, G.-S. Shieh, C.-C. Chang, Y.-T. Yo, C.-H. Su, M.-Y. Chang, Y.-H. Huang, P. Wu, and A.-L. Shiau Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models Clin. Cancer Res., February 15, 2008; 14(4): 1228 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Dong Zhang, H. Fouad, W. D. Zoma, S. A. Salama, M. J. Wentz, and A. Al-Hendy Expression of Stem and Germ Cell Markers Within Nonfollicle Structures in Adult Mouse Ovary Reproductive Sciences, February 1, 2008; 15(2): 139 - 146. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Jomary and S. E. Jones Induction of Functional Photoreceptor Phenotype by Exogenous Crx Expression in Mouse Retinal Stem Cells Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 429 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Webster, V. Yuzbasiyan-Gurkan, J. E. Trosko, C.-C Chang, and M. Kiupel Expression of the Embryonic Transcription Factor Oct4 in Canine Neoplasms: A Potential Marker for Stem Cell Subpopulations in Neoplasia Vet. Pathol., November 1, 2007; 44(6): 893 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Yoshida, M. Amanai, T. Fukui, E. Kajikawa, M. Brahmajosyula, A. Iwahori, Y. Nakano, S. Shoji, J. Diebold, H. Hessel, et al. Broad, ectopic expression of the sperm protein PLCZ1 induces parthenogenesis and ovarian tumours in mice Development, November 1, 2007; 134(21): 3941 - 3952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mimeault and S. K. Batra Interplay of distinct growth factors during epithelial mesenchymal transition of cancer progenitor cells and molecular targeting as novel cancer therapies Ann. Onc., October 1, 2007; 18(10): 1605 - 1619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lan, D. Cui, K. Nowka, and M. Derwahl Stem Cells Derived from Goiters in Adults Form Spheres in Response to Intense Growth Stimulation and Require Thyrotropin for Differentiation into Thyrocytes J. Clin. Endocrinol. Metab., September 1, 2007; 92(9): 3681 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Villadsen, A. J. Fridriksdottir, L. Ronnov-Jessen, T. Gudjonsson, F. Rank, M. A. LaBarge, M. J. Bissell, and O. W. Petersen Evidence for a stem cell hierarchy in the adult human breast J. Cell Biol., April 9, 2007; 177(1): 87 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hattori, Y. Imao, K. Nishino, N. Hattori, J. Ohgane, S. Yagi, S. Tanaka, and K. Shiota Epigenetic regulation of Nanog gene in embryonic stem and trophoblast stem cells Genes Cells, March 1, 2007; 12(3): 387 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jones and T. A. Libermann Genomics of Renal Cell Cancer: The Biology Behind and the Therapy Ahead Clin. Cancer Res., January 15, 2007; 13(2): 685s - 692s. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fujita, A. Tsujimura, Y. Miyagawa, H. Kiuchi, Y. Matsuoka, T. Takao, S. Takada, N. Nonomura, and A. Okuyama Isolation of Germ Cells from Leukemia and Lymphoma Cells in a Human In vitro Model: Potential Clinical Application for Restoring Human Fertility after Anticancer Therapy Cancer Res., December 1, 2006; 66(23): 11166 - 11171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Ahn, C.-C. Chang, and J. J. Schroeder Evaluation of sphinganine and sphingosine as human breast cancer chemotherapeutic and chemopreventive agents. Experimental Biology and Medicine, November 1, 2006; 231(10): 1664 - 1672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gupta, C. Verfaillie, D. Chmielewski, S. Kren, K. Eidman, J. Connaire, Y. Heremans, T. Lund, M. Blackstad, Y. Jiang, et al. Isolation and Characterization of Kidney-Derived Stem Cells J. Am. Soc. Nephrol., November 1, 2006; 17(11): 3028 - 3040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Redvers, A. Li, and P. Kaur Side population in adult murine epidermis exhibits phenotypic and functional characteristics of keratinocyte stem cells PNAS, August 29, 2006; 103(35): 13168 - 13173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Ling, M.-D. Kuo, C.-L. Li, A. L. Yu, Y.-H. Huang, T.-J. Wu, Y.-C. Lin, S.-H. Chen, and J. Yu Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro PNAS, June 20, 2006; 103(25): 9530 - 9535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yu, D. Fang, S. M. Kumar, L. Li, T. K. Nguyen, G. Acs, M. Herlyn, and X. Xu Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles Am. J. Pathol., June 1, 2006; 168(6): 1879 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Covello, J. Kehler, H. Yu, J. D. Gordan, A. M. Arsham, C.-J. Hu, P. A. Labosky, M. C. Simon, and B. Keith HIF-2{alpha} regulates Oct-4: effects of hypoxia on stem cell function, embryonic development, and tumor growth. Genes & Dev., March 1, 2006; 20(5): 557 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Matthai, R. Horvat, M. Noe, F. Nagele, A. Radjabi, M. van Trotsenburg, J. Huber, and A. Kolbus Oct-4 expression in human endometrium Mol. Hum. Reprod., January 1, 2006; 12(1): 7 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Leri, J. Kajstura, and P. Anversa Cardiac Stem Cells and Mechanisms of Myocardial Regeneration Physiol Rev, October 1, 2005; 85(4): 1373 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Leri and J. Kajstura Endothelial Progenitor Cells: Unexpected Disclosures Circ. Res., August 19, 2005; 97(4): 299 - 301. [Full Text] [PDF]
|
|
|
|
|
|
L. Patrawala, T. Calhoun, R. Schneider-Broussard, J. Zhou, K. Claypool, and D. G. Tang Side Population Is Enriched in Tumorigenic, Stem-Like Cancer Cells, whereas ABCG2+ and ABCG2- Cancer Cells Are Similarly Tumorigenic Cancer Res., July 15, 2005; 65(14): 6207 - 6219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. ZENDER, W. XUE, C. CORDON-CARDO, G.J. HANNON, R. LUCITO, S. POWERS, P. FLEMMING, M.S. SPECTOR, and S.W. LOWE Generation and Analysis of Genetically Defined Liver Carcinomas Derived from Bipotential Liver Progenitors Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 251 - 261. [Abstract] [PDF]
|
|