Carcinogenesis Advance Access originally published online on August 25, 2005
Carcinogenesis 2006 27(2):337-343; doi:10.1093/carcin/bgi218
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Carcinogenesis vol.27 no.2 © Oxford University Press 2005; all rights reserved.
Tetraploidy and chromosomal instability are early events during cervical carcinogenesis
1 Environmental Toxicology Graduate Program, Department of Cell Biology and Neuroscience, 5429 Boyce Hall, University of California, Riverside, CA-92521, USA, 2 Mexican National Cancer Institute, Mexico City, Mexico and 3 Department of Genomic Medicine and Environmental Toxicology, National Autonomous University of Mexico (UNAM), Mexico City, Mexico
* To whom correspondence should be addressed. Tel: (951) 827-4497; Fax: (951) 827-3087; Email: david.eastmond{at}ucr.edu
Chromosomal instability as manifested by increases in aneuploidy and structural chromosome aberrations is believed to play a critical role in the intermediate to late stages in the development of cervical malignancies. The current study was designed to determine the role of tetraploidy in the formation of aneuploidy and ascertain the occurrence of these alterations during the earlier stages of cervical carcinogenesis. Cervical cell samples, with diagnoses ranging from Normal to high-grade lesions, (HSIL) were obtained from 143 women and were evaluated for chromosomal alterations using dual-probe fluorescence in situ hybridization. Cervical cells from a subset of the group were also evaluated for chromosomal instability in the form of micronuclei. The frequencies of cells exhibiting either tetrasomy or aneusomy for Chromosomes 3 and 17 increased significantly with disease progression and displayed distinctive patterns where aneusomy was rarely present in the absence of tetrasomy. The frequencies of micronuclei that formed through either chromosomal loss or breakage increased significantly in both the low-grade and high-grade diagnostic categories and were highly correlated with both the number of tetrasomic and aneusomic cervical cells. In addition, a unique chromosomal alteration involving a significant non-random loss of Chromosome 17 specific to near-tetraploid aneusomic cells (trisomy 17 and tetrasomy 3) was observed. We conclude that tetraploidy and chromosomal instability are related events occurring during the early stages of cervical carcinogenesis that predispose cervical cells to the formation of aneuploidy frequently involving the loss of Chromosome 17.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Zhai, R. Kuick, B. Nan, I. Ota, S. J. Weiss, C. L. Trimble, E. R. Fearon, and K. R. Cho Gene Expression Analysis of Preinvasive and Invasive Cervical Squamous Cell Carcinomas Identifies HOXC10 as a Key Mediator of Invasion Cancer Res., November 1, 2007; 67(21): 10163 - 10172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, S. A. Heilman, D. Illanes, G. Sluder, and J. J. Chen p53-Independent Abrogation of a Postmitotic Checkpoint Contributes to Human Papillomavirus E6-Induced Polyploidy Cancer Res., March 15, 2007; 67(6): 2603 - 2610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bonassi, A. Znaor, M. Ceppi, C. Lando, W. P. Chang, N. Holland, M. Kirsch-Volders, E. Zeiger, S. Ban, R. Barale, et al. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans Carcinogenesis, March 1, 2007; 28(3): 625 - 631. [Abstract] [Full Text] [PDF]
|
|