Carcinogenesis Advance Access originally published online on March 8, 2006
Carcinogenesis 2006 27(9):1803-1811; doi:10.1093/carcin/bgl006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Caffeic acid suppresses UVB radiation-induced expression of interleukin-10 and activation of mitogen-activated protein kinases in mouse
Institute of BioAgricultural Sciences, Academia Sinica Taipei 115, Taiwan, Republic of China
*To whom correspondence should be addressed. Tel: +886 2 2651 5911; Fax: +886 2 2651 5693; Email: nsyang{at}gate.sinica.edu.tw
Ultraviolet B (UVB) radiation present in sunlight causes sustained immune suppression, photocarcinogenesis and photoaging in humans. Interleukin-10 (IL-10) plays a critical role in UVB-induced immune suppression by inhibiting cell-mediated immune reactions. Mitogen-activated protein kinases (MAPKs) have been implicated in UVB-induced skin carcinogenesis. Caffeic acid (CA), a phenolic acid present in many dietary plants has been shown to confer antioxidant, anti-inflammatory and anticancer activities. In this study, we evaluated the protective effects of CA against UVB radiation-induced IL-10 expression and phosphorylation of MAPKs in mouse skin. An in vivo transgenic IL-10 promoter–luciferase-reporter gene based assay revealed that CA inhibits the transcriptional activation of UVB-induced IL-10 promoter. This was further confirmed by significant inhibition of UVB radiation-induced IL-10 mRNA expression and protein production by CA in mouse skin. Contact hypersensitivity assay showed that CA could attenuate the local immune suppression induced by UVB radiation against a hapten, dinitrofluorobenzene. Our results indicated that CA might inhibit IL-10 production by interfering with an early step, prostaglandin E2 synthesis, in the activation of UVB-induced immune suppressive cytokine cascade. CA also significantly inhibited the UVB-induced activation of MAPK signal transduction pathways, such as extracellular signal-regulated protein kinase, c-Jun N-terminal protein kinase and p38 mitogen-activated protein kinase, and the downstream transcription factors activator protein-1 and nuclear factor-kappa B. The findings of our study suggest that CA may confer significant protection against UVB-induced immune suppression and photocarcinogenesis in vivo and provide the possible underlying molecular basis for its actions. Therefore, CA may have therapeutic potential as a topical protective agent against the deleterious effects of UVB radiation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. J. Kang, K. W. Lee, B. J. Shin, S. K. Jung, M. K. Hwang, A. M. Bode, Y.-S. Heo, H. J. Lee, and Z. Dong Caffeic acid, a phenolic phytochemical in coffee, directly inhibits Fyn kinase activity and UVB-induced COX-2 expression Carcinogenesis, February 1, 2009; 30(2): 321 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Black, J. P. Gray, M. P. Shakarjian, D. L. Laskin, D. E. Heck, and J. D. Laskin Distinct effects of ultraviolet B light on antioxidant expression in undifferentiated and differentiated mouse keratinocytes Carcinogenesis, January 1, 2008; 29(1): 219 - 225. [Abstract] [Full Text] [PDF]
|
|