Carcinogenesis Advance Access published online on August 1, 2003
Carcinogenesis, doi:10.1093/carcin/bgg129
© 2003 by Oxford University Press
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CANCER BIOLOGY
1 Department of Pediatrics, College of Medicine, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612; Depatment of Pathology, Jilin University, Changchun, Jilin, China
* Corresponding author. E-mail: sjlu{at}uic.edu.
Received 24 March 2003
; revised 24 June 2003
; accepted 23 July 2003
Rats, like humans, vary considerably in susceptibility for mammary cancer development among different strains. The Copenhagen (Cop) rat is extremely resistant to mammary cancer development induced by a variety of carcinogens. Multiple genetic loci have been linked to the resistant phenotype, but the genes have yet to be cloned and the mechanisms underlying the resistance still remain unknown. Transplantation experiments, however, have demonstrated that these genes act only in the epithelial cells of mammary parenchyma; they do not act systemically. In the present study, we analyzed genes differentially expressed in mammary epithelial cells obtained from pubescent female Cop and susceptible Buffalo (Buf) rats, using PCR-based suppressive subtractive hybridization and cDNA microarray approaches. Our results showed a high degree of similarity in the expression profiles of about 4,000 genes between Cop and Buf rats, with a few exceptions. We found that the interleukine-2 receptor
Identification of genes preferentially expressed in mammary epithelial cells of copenhagen rat using subtractive hybridization and microarrays
2 Department of Pediatrics, College of Medicine, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612; Cancer Center, College of Medicine, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612
(IL-2R) chain gene and claudin-6 gene were preferentially expressed in mammary epithelial cells purified from Cop rats. We further demonstrated that IL-2R message was undetectable in two rat mammary cancer cell lines and in two human breast cancer cell lines. The level of claudin-6 mRNA was undetectable in two rat mammary cancer cell lines and was lower in two human breast cancer cell lines and one breast cancer sample than that in normal breast tissues. These results suggest that IL-2R and claudin-6 may function as tumor suppressors, particularly for breast cancer. However, this possibility needs further investigation.
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