Skip Navigation



Carcinogenesis Advance Access published online on September 11, 2003
Carcinogenesis, doi:10.1093/carcin/bgg167
© 2003 by Oxford University Press
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
24/12/1913    most recent
bgg167v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zsivkovits, M.
Right arrow Articles by Knasmüller, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zsivkovits, M.
Right arrow Articles by Knasmüller, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 Oxford University Press

MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION

Prevention of heterocyclic amine induced DNA-damage in colon and liver of rats by different lactobacilli strains

Markus Zsivkovits 1, Kassie Fekadu 1, Gerhard Sontag 2, Ursula Nabinger 2, Wolfgang W. Huber 1, Michael Kundi 3, Asima Chakraborty 1, Helmuth Foissy 4, and Siegfried Knasmüller 1*

1 Institute of Cancer Research, Borschkegasse 8a, A-1090 Vienna, Austria
2 Institute of Analytical Chemistry, Waehringer Strasse 38, A-1090 Vienna, Austria
3 Institute of Environmental Hygiene, Kinderspitalgasse 15, A-1090 Vienna, Austria
4 Department of Diary Research and Microbiology, Agricultural University, Gregor Mendel Strasse 33, A-1180, Vienna, Austria

* Corresponding author. E-mail: siegfried.knasmueller{at}univie.ac.at.

Received 13 May 2003 ; revised 14 August 2003 ; accepted 20 August 2003

Abstract

Aim of the present study was the investigation of the impact of four different lactobacilli (LB) strains, namely Lactobacillus bulgaricus #291, Streptococcus thermophilus #F4, Streptococcus thermophilus V3 and Bifidobacterium longum #BB536, which are used for the production of yogurt on the DNA-damaging effects of heterocyclic aromatic amines (HCAs). Male F344 rats were treated orally with HCA-mixtures containing PhIP, MeIQx, DiMeIQx, A{alpha}C, and IQ, which were representative for the HCA distribution found in either fried beef ("beef-mix") or chicken ("chicken-mix"). Suspensions of the LB were given by gavage to the animals simultaneously with and at different time periods before the administration of the HCAs. Subsequently, the extent of DNA-migration was measured in colon and liver cells in single cell gel electrophoresis (SCGE) assays. All four strains, caused complete inhibition of DNA-damage induced with beef-mix after administration of 1 x 1010 LB cells/animal, whereas with chicken-mix only marginal (non-significant) effects were seen. The inhibition of beef induced DNA-damage was dose dependent and was still significant when 1 x 107 cells/animal were administered. Time kinetics studies showed that the protective effects were still significant, when the LB were given 12 h before the beef-mix. Comparison of the present results with chemical analytical data from in vitro experiments suggest that the strong reduction of DNA-migration seen in the animals can be only partly explained by direct binding effects. The results of the present study show that LB are highly protective towards the genotoxic effects of HCAs under conditions which are relevant for humans and provide a possible explanation for the reduced colon cancer rates observed in some studies in individuals with either high LB-counts in their feces or with a high consumption of LB-containing foods.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
C. Humblot, M. Murkovic, L. Rigottier-Gois, M. Bensaada, A. Bouclet, C. Andrieux, J. Anba, and S. Rabot
{beta}-Glucuronidase in human intestinal microbiota is necessary for the colonic genotoxicity of the food-borne carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline in rats
Carcinogenesis, November 1, 2007; 28(11): 2419 - 2425.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.