Skip Navigation



Carcinogenesis Advance Access published online on September 26, 2003
Carcinogenesis, doi:10.1093/carcin/bgg171
© 2003 by Oxford University Press
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
24/12/1929    most recent
bgg171v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zhang, H.
Right arrow Articles by Rosdahl, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, H.
Right arrow Articles by Rosdahl, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 Oxford University Press

CARCINOGENESIS

Ultraviolet A and B differently induce intracellular protein expression in human skin melanocytes - a speculation of separate pathways in initiation of melanoma

Hong Zhang 1* and Inger Rosdahl 1

1 Department of Dermatology, Institute of Biomedicine and Surgery, Linköping University, SE-581 85 Linköping, Sweden

* Corresponding author. E-mail: hong.zhang{at}ibk.liu.se.

Received 24 October 2002 ; revised 8 September 2003 ; accepted 9 September 2003

Abstract

Ultraviolet (UV) irradiation has been involved in both initiation and promotion of carcinogenesis in melanoma. Alterations of cellular proliferation-proteins, such as p73, Nup88, Id1 and p27 have been considered to play critical roles in melanoma development. However, the molecular mechanisms behind melanoma carcinogenesis are still poorly understood. In this study, we used human skin melanocytes as an experimental model system to investigate effects of UV irradiation on protein expression concerning cellular proliferation. The melanocytes prepared from human foreskin were separately exposed to various doses of UVA or UVB and post-cultivated for 24 or 48 hrs. Total proteins were isolated from the melanocytes, and expression of p73, Nup88, Id1, p27, bcl-2 and PCNA proteins was examined by Western blotting and immunocytochemistry. Results showed that expression of p73 and Nup88 was enhanced by UVA irradiation in a dose- and time-dependent manner. However, expression of Id1, p27, bcl-2 and PCNA proteins was not changed upon exposure to the UVA. Id1 and p27 proteins were overexpressed by exposure to UVB, but expression of p73, Nup88, bcl-2 and PCNA proteins was not changed by the UVB irradiation. These data suggested that UVA and UVB irradiation might lead to alterations of the different intracellular proteins. UVA enhanced protein expression concerning cell growth (p73 and Nup88) and UVB might overexpress proteins concerning cellular proliferation (Id1 and p27). UVA and UVB may induce initiation of melanoma via separate intracellular pathways.

p73, Nup88, Id1, p27, UVA, UVB, melanocytes
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol Ind HealthHome page
R. M Brand, J. Pike, R. M Wilson, and A. R Charron
Sunscreens containing physical UV blockers can increase transdermal absorption of pesticides
Toxicology and Industrial Health, February 1, 2003; 19(1): 9 - 16.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.