Skip Navigation



Carcinogenesis Advance Access published online on December 4, 2003
Carcinogenesis, doi:10.1093/carcin/bgh030
© 2003 by Oxford University Press
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
25/3/439    most recent
bgh030v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Suzuki, S.
Right arrow Articles by Shirai, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, S.
Right arrow Articles by Shirai, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 Oxford University Press

CARCINOGENESIS

Specific differences in gene expression profile revealed by cDNA microarray analysis of glutathione S-transferase placental form (GST-P) immunohistochemically positive rat liver foci and surrounding tissue

Shugo Suzuki 1*, Makoto Asamoto 1, Kazunari Tsujimura 2, and Tomoyuki Shirai 1

1 Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, Aichi, Japan
2 Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, Aichi, Japan; Chemicals Evaluation and Research Institute, Chemicals Assessment center, Saitama, Japan

* Corresponding author. E-mail: shugo{at}med.nagoya-cu.ac.jp.

Received 8 July 2003 ; revised 7 November 2003 ; accepted 15 November 2003

Abstract

Glutathione S-transferase placental form (GST-P), one of the glutathione S-transferases family of detoxification enzymes, is a very useful marker of rat liver preneoplastic lesions. We here investigated the gene expression profile in GST-P positive foci as compared with surrounding GST-P negative areas in the same liver of rats treated with diethylnitrosamine (DEN) and then 2-acetylaminofluorene (2-AAF) combined with partical hepatectomy. GST-P positive foci were harvested by laser microdissection and total RNAs were extracted to allow gene expression profiles to be assessed by cDNA microarray assays. Transaldolase, rat aflatoxin B1 aldehyde reductase (AFAR) and gamma-glutamylcysteine synthetase (GCS) were found as up-regulated genes and regucalcin as a down-regulated gene, in line with findings for hepatocellular carcinomas. The results indicate that the approach adopted is useful for understanding mechanisms of hepatocarcinogenesis and identification of new markers for rat liver preneoplastic foci.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
J. Nishimura, Y. Dewa, T. Okamura, M. Jin, Y. Saegusa, M. Kawai, T. Umemura, M. Shibutani, and K. Mitsumori
Role of Nrf2 and Oxidative stress on Fenofibrate-Induced Hepatocarcinogenesis in Rats
Toxicol. Sci., December 1, 2008; 106(2): 339 - 349.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Gironella, M. Seux, M.-J. Xie, C. Cano, R. Tomasini, J. Gommeaux, S. Garcia, J. Nowak, M. L. Yeung, K.-T. Jeang, et al.
Tumor protein 53-induced nuclear protein 1 expression is repressed by miR-155, and its restoration inhibits pancreatic tumor development
PNAS, October 9, 2007; 104(41): 16170 - 16175.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Gommeaux, C. Cano, S. Garcia, M. Gironella, S. Pietri, M. Culcasi, M.-J. Pebusque, B. Malissen, N. Dusetti, J. Iovanna, et al.
Colitis and Colitis-Associated Cancer Are Exacerbated in Mice Deficient for Tumor Protein 53-Induced Nuclear Protein 1
Mol. Cell. Biol., March 15, 2007; 27(6): 2215 - 2228.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
D. von Smolinski, M. Blessenohl, C. Neubauer, K. Kalies, and A. Gebert
Validation of a Novel Ultra-Short Immunolabeling Method for High-Quality mRNA Preservation in Laser Microdissection and Real-Time Reverse Transcriptase-Polymerase Chain Reaction
J. Mol. Diagn., May 1, 2006; 8(2): 246 - 253.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.