Carcinogenesis Advance Access published online on December 4, 2003
Carcinogenesis, doi:10.1093/carcin/bgh033
© 2003 by Oxford University Press
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MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION
1 Molecular and Nutritional Epidemiology Unit and Cancer Risk Factor Branch, Molecular Biology Laboratory - CSPO, Scientific Institute of Tuscany, Florence, Italy
* Corresponding author. E-mail: d.palli{at}cspo.it.
Received 25 September 2003
; revised 5 November 2003
; accepted 15 November 2003
Frequent consumption of fresh fruit and vegetables and polymorphisms in the detoxifying enzyme glutathione S-transferase M1 (GSTM1) and other metabolic genes have been shown to modulate cancer risk at some sites. We have recently shown that DNA adducts, a reliable indicator of genotoxic damage and, possibly, of cancer risk, are modulated by plasma levels of selected micronutrients (Palli et al., Carcinogenesis, 24:739-46, 2003). Here we further investigate the association between DNA adduct levels and consumption of major food groups and foods, and the estimated dietary intake of nutrients, taking into account the possible modifying effect of metabolic polymorphisms, in a larger sample of 634 healthy adults enrolled in a prospective study in Italy. DNA adducts and 5 polymorphic metabolic genotypes (GSTM1, GSTT1, NAT2, CYP1A1 and MTHFR) were determined in peripheral leukocytes by using 32P-postlabeling technique and PCR methods. DNA bulky adducts (mean: 7.82±0.40 per 109 nucleotides) were detected in 482/634 samples (76.0%). Overall, DNA adduct levels were significantly and inversely associated with the intake of raw leafy vegetables (p=0.02), non-citrus fruits (p=0.04), potassium (p=0.01) and Our results suggest that the role of a diet rich in antioxidants in preventing or reducing DNA adduct formation is restricted to subjects lacking the detoxifying activity of GSTM1 isoenzyme (approximately 50% of the general population).
The effects of diet on DNA bulky adduct levels are strongly modified by GSTM1 genotype: a study on 634 subjects
2 Genetics Research Institute, Milan, Italy
3 Epidemiology Unit, INT, Milan, Italy
4 Department of Clinical and Experimental Medicine, Federico II University, Naples, Italy
5 Registro Tumori, A.O. "Civile-M.P. Arezzo", Ragusa, Italy
6 Cancer Epidemiology Unit, CPO, Turin, Italy
-carotene (p=0.05). No association was evident with the 5 genotypes. Stratification by GSTM1 genotype showed strong inverse associations of DNA adduct levels with increasing consumption of all vegetables combined (p=0.04), leafy vegetables (p=0.004), raw leafy vegetables (p=0.002), and fish (p=0.03) among 307 GSTM1-null subjects; strong inverse associations also emerged with estimated dietary intakes of -carotene (p=0.004), vitamin E (p=0.004), niacin (p=0.02) and potassium (p=0.01). In contrast, no association emerged among 295 subjects with a GSTM1-wild genotype. Overall, statistically significant interactions in predicting DNA adduct levels were observed between the GSTM1-null genotype and consumption of leafy vegetables (p=0.01), white meat (p=0.04), and intake of vitamin C (p=0.04), vitamin E (p=0.05) and -carotene (p=0.02).
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