Carcinogenesis Advance Access published online on January 16, 2004
Carcinogenesis, doi:10.1093/carcin/bgh065
© 2004 by Oxford University Press
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MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION
1 Lombardi Cancer Center and Department of Oncology, Georgetown University, 3970 Reservoir Rd, NW, Washington, DC 20007, USA
* Corresponding author. E-mail: Clarkel{at}georgetown.edu.
Received 1 October 2003
; revised 26 November 2003
; accepted 16 December 2003
Prepubertal exposure to soy or its biologically active component genistein reduces later breast cancer risk both in animal models and human populations. We investigated whether that might be due to reported estrogenic properties of genistein. Our study indicated that daily prepubertal exposures between postnatal days 7 and 20 to 10 µg of 17-
Estradiol, Genistein, BRCA1, Estrogen receptor Prepubertal estradiol and genistein exposures up-regulate BRCA1 mRNA and reduce mammary tumorigenesis
2 Department of Medical Nutrition, Karolinska Institute, Huddinge 14186, Sweden
estradiol (E2) reduced later risk of developing 7,12-dimethylbenz[a]athracene (DMBA) induced mammary tumors. Assessment of mammary gland morphology revealed that both prepubertal E2 and genistein (50 µg daily) exposures reduced the size of mammary epithelial area and number of terminal end buds (TEBs), and increased the density of lobulo-alveolar structures, suggesting that these exposures induced elimination of targets for malignant transformation by differentiation. Next, the mechanisms mediating the protective effects of E2 and genistein were investigated. E2 is shown to up-regulate BRCA1, a tumor suppressor gene that participates in DNA damage repair processes and cell differentiation, and that down-regulates the activity of the estrogen receptor (ER)-
. The expression of BRCA1 mRNA was up-regulated in the mammary glands of rats exposed to E2 or genistein during prepuberty, when determined at the ages of 3, 8 and 16 weeks. Prepubertal E2 exposure reduced ER- levels in the mammary gland, while prepubertal genistein exposure had an opposite effect. Our results suggest that prepubertal estrogenic exposures may reduce later breast cancer risk by inducing a persistent up-regulation of BRCA1 in the mammary gland., Estrogen receptor , Mammary gland, Prepuberty, Mammary tumorigenesis
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