Carcinogenesis Advance Access published online on February 19, 2004
Carcinogenesis, doi:10.1093/carcin/bgh123
© 2004 by Oxford University Press
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION
1 Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Jouy-en-Josas, France
* Corresponding author. E-mail: druesne{at}jouy.inra.fr.
Received 23 December 2003
; revised 9 February 2004
; accepted 10 February 2004
Diallyl disulfide (DADS) is a naturally occurring organosulfur compound, from garlic, which exerts pleiotropic biological effects. In rodents, DADS inhibits colon chemically-induced carcinogenesis. DADS anti-promoting effect may partly result from its ability to inhibit tumoral cell proliferation in vivo and in vitro. As far as DADS may modulate the expression of a subset of genes, we investigated DADS effect on histone acetylation, in two human colon tumor cell lines. Our study demonstrates that in Caco-2 and HT-29 cells treated for 6 h, 200 µM DADS increases histone H3 acetylation (x2 and x1.4 respectively). In Caco-2 cells, we also observed histone H4 hyperacetylation, preferentially at the lysine residues 12 and 16. We explored the effects of DADS and one of its metabolites, allyl mercaptan (AM), on histone deacetylase (HDAC) activity: using nuclear extracts of Caco-2 cells, 200 µM DADS decreased HDAC activity by 29% and AM at the same concentration was more efficient (92% inhibition). We also observed that DADS induced an increase in p21waf1/cyp1 expression, at mRNA and protein levels, in both cell lines. This effect was associated with an accumulation of cells in the G2 phase of the cell cycle. Our results suggest that in Caco-2 and HT-29 cells, DADS could inhibit cell proliferation through the inhibition of HDAC activity, histone hyperacetylation and increase in p21waf1/cyp1 expression. The present study provides evidence for cellular and molecular responses triggered by DADS that could be linked to its effect on histone acetylation and play a role in its protective properties on colon carcinogenesis.
Diallyl disulfide (DADS) increases histone acetylation and p21waf1/cyp1 expression in human colon tumor cell lines
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Nian, B. Delage, J. T. Pinto, and R. H. Dashwood Allyl mercaptan, a garlic-derived organosulfur compound, inhibits histone deacetylase and enhances Sp3 binding on the P21WAF1 promoter Carcinogenesis, September 1, 2008; 29(9): 1816 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shankar, Q. Chen, S. Ganapathy, K. P. Singh, and R. K. Srivastava Diallyl trisulfide increases the effectiveness of TRAIL and inhibits prostate cancer growth in an orthotopic model: molecular mechanisms Mol. Cancer Ther., August 1, 2008; 7(8): 2328 - 2338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. T. Ngo, D. B. Williams, L. Cobiac, and R. J. Head Does Garlic Reduce Risk of Colorectal Cancer? A Systematic Review J. Nutr., October 1, 2007; 137(10): 2264 - 2269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. K. Hagos, R. E. Carroll, T. Kouznetsova, Q. Li, V. Toader, P. A. Fernandez, S. M. Swanson, and G. R.J. Thatcher Colon cancer chemoprevention by a novel NO chimera that shows anti-inflammatory and antiproliferative activity in vitro and in vivo Mol. Cancer Ther., August 1, 2007; 6(8): 2230 - 2239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zhang, M.-L. Noordine, C. Cherbuy, P. Vaugelade, J. M. Pascussi, P.-H. Duee, and M. Thomas Different activation patterns of rat xenobiotic metabolism genes by two constituents of garlic Carcinogenesis, October 1, 2006; 27(10): 2090 - 2095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Myzak, K. Hardin, R. Wang, R. H. Dashwood, and E. Ho Sulforaphane inhibits histone deacetylase activity in BPH-1, LnCaP and PC-3 prostate epithelial cells Carcinogenesis, April 1, 2006; 27(4): 811 - 819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Milner Preclinical Perspectives on Garlic and Cancer J. Nutr., March 1, 2006; 136(3): 827S - 831S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Pinto, B. F. Krasnikov, and A. J. L. Cooper Redox-Sensitive Proteins Are Potential Targets of Garlic-Derived Mercaptocysteine Derivatives J. Nutr., March 1, 2006; 136(3): 835S - 841S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Katsuki, K. Hirata, H. Ishikawa, N. Matsuura, S.-i. Sumi, and H. Itoh Aged Garlic Extract Has Chemopreventative Effects On 1,2-Dimethylhydrazine-Induced Colon Tumors in Rats J. Nutr., March 1, 2006; 136(3): 847S - 851S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Dashwood, M. C. Myzak, and E. Ho Dietary HDAC inhibitors: time to rethink weak ligands in cancer chemoprevention? Carcinogenesis, February 1, 2006; 27(2): 344 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-J. Yang and S. Gregoire Class II Histone Deacetylases: from Sequence to Function, Regulation, and Clinical Implication Mol. Cell. Biol., April 15, 2005; 25(8): 2873 - 2884. [Full Text] [PDF]
|
|