Carcinogenesis Advance Access published online on July 29, 2004
Carcinogenesis, doi:10.1093/carcin/bgh238
© 2004 by Oxford University Press
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
* To whom correspondence should be addressed. E-mail: Pieter.vantveer{at}wur.nl.
Background: The glutathione (GSH)/glutathione S-transferase (GST) system is an important detoxification system in the gastrointestinal tract. A high activity of this system may benefit cancer prevention. Aim: To assess whether habitual consumption of fruits and vegetables, especially citrus fruits and brassica and allium vegetables, is positively associated with parameters reflecting the activity of the GSH/GST enzyme system in human rectal mucosa. Methods: GST enzyme activity, GST isoenzyme levels of GST-alpha (A1-1, A1-2 and A2-2), -mu (M1-1) and -pi (P1-1), and GSH levels were measured in rectal biopsies from 94 subjects. Diet, lifestyle, GSTM1 and GSTT1 null polymorphisms were assessed. Results: Mean GST enzyme activity was 237 nmol/min/mg protein (SD=79). Consumption of citrus fruits was positively associated with GST enzyme activity (difference between high and low consumption: 28.9 [95% CI=9.3-48.6] nmol/min/mg protein), but was not associated with the other parameters. A positive association with brassica vegetables was found among carriers of the GSTM1-plus genotype (difference between high and low consumption: 22.6 [95% CI=0.2-45.0] nmol/min/mg protein), but not among GSTM1-null individuals (-25.8 nmol/min/mg protein, 95% CI=-63.3-11.8). This is in line with a positive association between consumption of brassica vegetables and GST-mu isoenzyme level (difference between high and low consumption: 67.5%, 95% CI = [6.8-162.7]). Consumption of allium vegetables was not associated with GST enzyme activity, but negatively with GSTP1-1 levels (difference between high and low consumption: -23.3%, 95% CI = [-35.5; -8.6]). Associations were similar among those with the GSTT1-plus and GSTT1-null genotype. Conclusion: Variations in habitual consumption of fruits, particularly citrus fruits, and of vegetables, in particular brassica vegetables, among those with the GSTM1-plus genotype, may contribute to variations in human rectal GST enzyme activity.
Revised June 30, 2004
Accepted July 18, 2004
CARCINOGENESIS
Habitual consumption of fruits and vegetables: associations with human rectal glutathione S-transferase
2 Department of Gastroenterology and Hepatology, University Medical Centre Nijmegen, Nijmegen, The Netherlands
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Tijhuis, M. H.P.W. Visker, J. M.M.J.G. Aarts, W. H.M. Peters, H. M.J. Roelofs, L. O. d. Camp, I. M.C.M. Rietjens, A.-M. J.F. Boerboom, F. M. Nagengast, F. J. Kok, et al. Glutathione S-transferase phenotypes in relation to genetic variation and fruit and vegetable consumption in an endoscopy-based population Carcinogenesis, April 1, 2007; 28(4): 848 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ahn, M. D. Gammon, R. M. Santella, M. M. Gaudet, J. A. Britton, S. L. Teitelbaum, M. B. Terry, A. I. Neugut, S. M. Eng, Y. Zhang, et al. Effects of glutathione S-transferase A1 (GSTA1) genotype and potential modifiers on breast cancer risk Carcinogenesis, September 1, 2006; 27(9): 1876 - 1882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vanamala, T. Leonardi, B. S. Patil, S. S. Taddeo, M. E. Murphy, L. M. Pike, R. S. Chapkin, J. R. Lupton, and N. D. Turner Suppression of colon carcinogenesis by bioactive compounds in grapefruit Carcinogenesis, June 1, 2006; 27(6): 1257 - 1265. [Abstract] [Full Text] [PDF]
|
|