Skip Navigation



Carcinogenesis Advance Access published online on September 9, 2004
Carcinogenesis, doi:10.1093/carcin/bgh279
© 2004 by Oxford University Press
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
26/1/11    most recent
bgh279v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Xia, H. H.-X.
Right arrow Articles by Wong, B. C.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xia, H. H.-X.
Right arrow Articles by Wong, B. C.-Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received December 9, 2003
Revised February 3, 2004
Accepted August 31, 2004

CANCER BIOLOGY

Expression of macrophage migration inhibitory factor in esophageal squamous cell carcinoma and effects of bile acids and NSAIDs

Harry Hua-Xiang Xia 1, Shu Tian Zhang 2, Shiu Kum Lam 1, Marie Chia-Mi Lin 3, Hsiang Fu Kung 3, and Benjamin Chun-Yu Wong 1*

1 Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong
2 Department of Medicine, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing, China
3 Institute of Molecular Biology, The University of Hong Kong, Hong Kong

* To whom correspondence should be addressed. E-mail: bcywong{at}hku.hk.


  Abstract

Objectives: The aim of this study was to determine macrophage migration inhibitory factor (MIF) protein and mRNA expression in esophageal squamous cell carcinoma (ESCC), and the effect of bile acids, aspirin and a selective cyclooxygenase-2 (COX-2) inhibitor, NS398, on MIF expression in ESCC cells in vitro.

Methods: Specimens from tumors and the adjacent non-cancerous tissues were obtained from 52 ESCC patients. Western blotting was used for the detection of MIF protein expression, and reverse transcription-polymerase chain reaction (RT-PCR) for MIF mRNA expression. Cells of an ESCC cell line, Eca-109, were treated with chenodeoxycholate (CD, 100 µM), glycochenodeoxycholate (GCD, 1 mM), aspirin (1 mM), or NS398 (1 µM). Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect expression of MIF protein and mRNA, respectively, in the supernatant and cultured cells.

Results: Western blotting demonstrated that levels of MIF protein were increased in tumors vs. non-malignant tissues, with the expression ratio of MIF over {beta}-actin of 0.93±0.21 and 0.57±0.08, respectively (P=0.012). In vitro, both CD and GCD induced a dramatic increase in MIF protein and mRNA in ESCC cells. On the other hand, aspirin and NS398 significantly decreased MIF protein and mRNA expression, and completely blocked bile acid-induced MIF synthesis in the presence or absence of prostaglandin E2.

Conclusion: MIF expression is increased in ESCC. Whereas bile acids induce MIF expression in ESCC cells, aspirin and NS398 significantly inhibit MIF expression, even in the presence of bile acids, via a COX-independent mechanism.

Keywords: Macrophage migration inhibitory factor; esophageal squamous cell carcinoma; bile acids; aspirin; NS398.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.