Carcinogenesis Advance Access published online on October 28, 2004
Carcinogenesis, doi:10.1093/carcin/bgh321
© 2004 by Oxford University Press
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1 National Food Safety Toxicology Center, Department of Pediatrics and Human Development, Michigan State University, East Lansing, MI 48824; Department of Physiology, Michigan State University, East Lansing, MI 48824
* To whom correspondence should be addressed. The Oct 3/4 gene, a POU family transcription factor, has been noted as being specifically expressed in embryonic stem cells, in tumor cells but not in cells of differentiated tissues. With the ability to isolate adult human stem cells, it became possible to test Oct3/4 gene in stem cells and to test the stem cell theory of carcinogenesis. Using antibodies and PCR primers, we tested human breast, liver, pancreas, kidney, mesenchyme, and gastric stem cells, HeLa and MCF-7 cancer cell lines, as well as human, dog and rat tumors for Oct4 expression. The results indicated that human adult stem cells, immortalized non-tumorigenic cells, tumor cells and cell lines, but not differentiated cells, expressed Oct4. Oct4 was expressed in a few cells found in the basal layer of human skin epidermis. The data demonstrate that adult stem cells maintain the expression of Oct4, consistent with the stem cell hypothesis of carcinogenesis.
Revised October 14, 2004
Accepted October 16, 2004
CARCINOGENESIS
Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis
2 National Food Safety Toxicology Center, Department of Pediatrics and Human Development, Michigan State University, East Lansing, MI 48824
3 Department of Physiology, Michigan State University, East Lansing, MI 48824
James E. Trosko, E-mail: james.trosko{at}ht.msu.edu
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