Carcinogenesis Advance Access published online on June 1, 2005
Carcinogenesis, doi:10.1093/carcin/bgi147
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1 The Institute of Environmental and Human Health and Department of Environmental Toxicology, Texas Tech University, Lubbock, Texas
* To whom correspondence should be addressed. Modulation of urinary excretion of green tea polyphenols (GTP) and oxidative DNA damage biomarker, 8-hydroxydeoxyguanosine (8-OHdG), was assessed in urine samples collected from a randomized, double blinded, and placebo controlled phase IIa chemoprevention trial with GTP in 124 individuals. These individuals were sero-positive for both HBsAg and aflatoxin-albumin adducts, and took GTP capsules daily at doses of 500-mg, 1,000-mg or a placebo for 3 months. Twenty-four hour urine samples were collected before the intervention and at the first and third month of the study. Urinary excretion of 8-OHdG and GTP components was measured by HPLC-CoulArray electrochemical detection. The baseline levels of 8-OHdG and GTP components among the three groups showed homogeneity (p>0.70), and a non-significant fluctuation was observed in the placebo group over the 3 months (p>0.30). In GTP treated groups, epigallocatechin (EGC) and epicatechin (EC) levels displayed significant and dose-dependent increases in both the 500-mg group and 1000-mg group (p<0.05). The 8-OHdG levels did not differ between the three groups at the 1-month collection, with medians of 1.83, 2.08, and 1.86 ng/mg-creatinine for placebo, 500-mg, and 1000-mg group, respectively (p=0.999). At the end of the 3 months' intervention, 8-OHdG levels decreased significantly in both GTP treated groups, with medians of 2.02, 1.03, and 1.15 ng/mg-creatinine for placebo, 500-mg, and 1000-mg group, respectively (p=0.007). These results suggest that urinary excretions of EGC and EC can serve as practical biomarkers for green tea consumption in human populations. The results also suggest that chemoprevention with GTP is effective in diminishing oxidative DNA damage.
Received March 4, 2005
Revised May 13, 2005
Accepted May 29, 2005
MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION
Phase IIa chemoprevention trial of green tea polyphenols in high-risk individuals of liver cancer: modulation of urinary excretion of green tea polyphenols and 8-hydroxydeoxyguanosine
2 The Institute of Environmental and Human Health and Department of Environmental Toxicology, Texas Tech University, Lubbock, Texas; Guangxi Cancer Institute, Nanning, Guangxi, China
3 Guangxi Cancer Institute, Nanning, Guangxi, China
4 Fusui Liver Cancer Institute, Fusui, Guangxi, China
5 Department of Mathematics and Statistics, Texas Tech University, Lubbock, Texas
Jia-Sheng Wang, E-mail: js.wang{at}ttu.edu
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