Carcinogenesis Advance Access published online on November 28, 2005
Carcinogenesis, doi:10.1093/carcin/bgi287
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1 Dipartimento di Medicina Sperimentale, Plesso Biotecnologico Integrato, Universita' di Parma, Via Volturno 39 - 43100 Parma, Italy
* To whom correspondence should be addressed. We previously found that human prostate cancer (CaP) progression is accompanied by differential expression of a panel of 8 informative genes, some of which are metabolically related. Gene profiling focused on this 8-genes pack by Northern blot analysis in combination with standard clinical information provided reliable prognostic prediction of human CaP. For a better insight into the potential of this 8-genes signature in tumor detection/classification and therapeutic response, we determined, by qPCR, the expression of these informative genes in the TRAMP mice model of CaP progression. The 8-genes signature resulted effective in discriminating, by linear discriminant analysis (LDA), the prostate of wild type mice from transgenic TRAMP mice developing CaP (p < 0.0002). Since it is known that Green Tea Catechins (GTCs) administration to TRAMP mice results in a substantial delay of CaP progression in 80% of the animals, while 20% remain unresponsive, we determined the 8-genes signature in the prostates of GTCs-sensitive and GTCs-resistant mice. LDA discriminated benign tissue from CaP (i.e. wild type + chemoprevented, GTCs-sensitive TRAMP mice, in which CaP progression was delayed, was discriminated from TRAMP mice + GTCs-resistant TRAMP mice, in which CaP developed irrespectively from GTCs administration; p < 0.01). Moreover, GTCs-sensitive TRAMP mice bearing CaP were discriminated from GTCs-resistant ones, (p = 0.0001). These results show that qPCR gene profiling, based on the signature of the 8-genes selected by us, could represent an appropriate means for studying the biological behavior of CaP, which may lead to identifying new tools of potential prognostic value, in that a molecular classification for presence/absence of cancer and for discriminating GTCs-responsive from GTCs-resistant CaPs is provided.
Received June 11, 2005
Revised November 11, 2005
Accepted November 22, 2005
MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION
Molecular classification of green tea catechins-sensitive and -resistant prostate cancer in the TRAMP mice model by quantitative real-time PCR gene profiling
Maurizio Scaltriti 1,
Lucia Belloni 1,
Andrea Caporali 1,
Pierpaola Davalli 2,
Daniel Remondini 3,
Federica Rizzi 1,
Serenella Astancolle 2,
Arnaldo Corti 2,
and
Saverio Bettuzzi 1 *
2 Dipartimento di Scienze Biomediche, Universita' di Modena e Reggio Emilia, Via G. Campi 287 - 41100 Modena, Italy
3 Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali (DIMORFIPA), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy
Saverio Bettuzzi, E-mail: saverio.bettuzzi{at}unipr.it
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