Skip Navigation



Carcinogenesis Advance Access published online on December 12, 2005
Carcinogenesis, doi:10.1093/carcin/bgi311
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
27/8/1526    most recent
bgi311v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Besaratinia, A.
Right arrow Articles by Pfeifer, G. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Besaratinia, A.
Right arrow Articles by Pfeifer, G. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org
Received October 13, 2005
Revised November 15, 2005
Accepted December 6, 2005

REVIEW

Investigating human cancer etiology by DNA lesion footprinting and mutagenicity analysis

Ahmad Besaratinia 1 * and Gerd P. Pfeifer 1

1 Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA

* To whom correspondence should be addressed.
Ahmad Besaratinia, E-mail: ania{at}coh.org


  Abstract

Many genotoxic carcinogens are known to leave unique signatures on cancer-related genes. The signature of carcinogens is manifested by the induction of characteristic mutations at distinctive nucleotide positions along oncogenes and/or tumor suppressor genes. Often, the nucleotide positions, wherein mutations occur, co-localize with the sites of initial DNA damage induced by the respective carcinogens. Thus, DNA damage-targeted mutation can be a predictor of carcinogenicity of genotoxins. Today, genomic sequencing technologies for investigating human cancer etiology are based on DNA-lesion footprinting in conjunction with mutagenicity analysis of genotoxic carcinogens. In this review article, we discuss the ligation-mediated polymerase chain reaction (LM-PCR) and terminal transferase-dependent (TD)-PCR, two versatile DNA-lesion footprinting techniques. We highlight the in vitro shuttle vector-based mutation systems for investigating site-specific mutagenicity of carcinogens and the in vivo transgenic rodent mutation systems for exploring DNA damaging and mutagenic properties of carcinogens. We present examples of application of each of these methodologies to human cancer etiology, and provide prospective views on investigations using these technologies for carcinogenicity testing.

Keywords: Cancer; DNA damage; footprinting; mutations; TP53 gene; ultraviolet radiation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-H. Yoon, L. Prakash, and S. Prakash
Highly error-free role of DNA polymerase {eta} in the replicative bypass of UV-induced pyrimidine dimers in mouse and human cells
PNAS, October 27, 2009; 106(43): 18219 - 18224.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. Besaratinia, S.-i. Kim, and G. P. Pfeifer
Rapid repair of UVA-induced oxidized purines and persistence of UVB-induced dipyrimidine lesions determine the mutagenicity of sunlight in mouse cells
FASEB J, July 1, 2008; 22(7): 2379 - 2392.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S.-i. Kim, G. P. Pfeifer, and A. Besaratinia
Lack of Mutagenicity of Acrolein-Induced DNA Adducts in Mouse and Human Cells
Cancer Res., December 15, 2007; 67(24): 11640 - 11647.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
V. M. Arlt, M. Stiborova, J. vom Brocke, M. L. Simoes, G. M. Lord, J. L. Nortier, M. Hollstein, D. H. Phillips, and H. H. Schmeiser
Aristolochic acid mutagenesis: molecular clues to the aetiology of Balkan endemic nephropathy-associated urothelial cancer
Carcinogenesis, November 1, 2007; 28(11): 2253 - 2261.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
A. Besaratinia and G. P. Pfeifer
A review of mechanisms of acrylamide carcinogenicity
Carcinogenesis, March 1, 2007; 28(3): 519 - 528.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.