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Carcinogenesis Advance Access published online on February 20, 2006
Carcinogenesis, doi:10.1093/carcin/bgi333
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org
Received October 7, 2005
Revised December 23, 2005
Accepted December 24, 2005

CANCER BIOLOGY

Detection of microsatellite instability in endometrial cancer: advantages of a panel of five mononucleotide repeats over the National Cancer Institute panel of markers

Yick Fu Wong 1, Tak Hong Cheung 1, Keith Wing Kit Lo 1, So Fan Yim 1, Loucia Kit Yin Chan 1, Olivier Buhard 2, Alex Duval 2, Tony Kwok Hung Chung 1, and Richard Hamelin 2 *

1 Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T., Hong Kong
2 INSERM U762, 75010, Paris, France

* To whom correspondence should be addressed.
Richard Hamelin, E-mail: richard.hamelin{at}cephb.fr


  Abstract

The aim of this study was to find the optimal set of microsatellite markers for diagnosis of the microsatellite instability (MSI) phenotype in endometrial cancers. We compared the sensitivity, specificity and ease of use of a reference panel of 5 markers originally recommended by the National Cancer Institute (NCI) for colorectal cancer and a panel of 5 quasimonomophic mononucleotide repeat markers (pentaplex PCR system). We used these panels to establish the MSI status of a series of 80 sporadic endometrial adenocarcinomas by comparing the allelic profiles of the markers between tumor and matching germline DNA. Both panels detected the same subset of 21/80 (26%) endometrial MSI carcinomas. However, in the MSI cases, the mean instability of the 5 mononucleotide repeats was 96.1% as compared to a mean instability of 69.8% for the 3 dinucleotide repeats of the NCI panel, indicating a superiority of mononucleotide repeats over dinucleotide repeats in detecting microsatellite instability. The fact that the two panels of markers detect the same set of MSI tumors is due to the presence of 2 mononucleotide repeats within the NCI panel. As previously demonstrated in gastric and colon MSI cases, the pentaplex PCR reaction using mononucleotide repeats is thus an easier and more sensitive method than the NCI panel, for the screening of MSI status in endometrial tumors.

Keywords: Microsatellite instability; endometrial cancer.
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