Skip Navigation



Carcinogenesis Advance Access published online on August 2, 2006
Carcinogenesis, doi:10.1093/carcin/bgl127
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
28/2/321    most recent
bgl127v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Pierre, F.
Right arrow Articles by Petit, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pierre, F.
Right arrow Articles by Petit, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org
Received August 8, 2005
Revised June 13, 2006
Accepted June 13, 2006

CANCER BIOLOGY

Apc mutation induces resistance of colonic cells to lipoperoxide-triggered apoptosis induced by faecal water from haem-fed rats

F. Pierre 1 *, S. Tache 1, F. Guéraud 2, A. L. Rerole 1, M.-L. Jourdan 3, and C. Petit 1

1 Ecole Nationale Vétérinaire Toulouse, UMR INRA-ENVT Xénobiotiques, 23 chemin des Capelles, 31076 Toulouse, France
2 Institut National de la Recherche Agronomique, UMR INRA-ENVT Xénobiotiques, 180 chemin de Tournefeuille, 31931 Toulouse, France
3 Inserm E 0211, Nutrition Croissance et Cancer, 10 Boulevard Tonnellé, 37032 Tours Cedex, France

* To whom correspondence should be addressed.
F. Pierre, E-mail: f.pierre{at}envt.fr


  Abstract

Recent epidemiological studies suggest that high meat intake is associated with promotion of colon cancer linked to haem-iron intake. We previously reported that dietary haem, in the form of either haemoglobin or meat, promotes precancerous lesions in the colon of rats given a low-calcium diet. The mechanism of promotion by haem is not known, but is associated with increased lipid peroxidation in faecal water and strong cytotoxic activity of faecal water on a cancerous mouse colonic epithelial cell line. To better understand the involvement of faecal water components of haem-fed rats in colon cancer promotion, we explored the effect of faecal water on normal (Apc +/+) or premalignant cells (Apc Min/+). Further, we tested if this effect was correlated to lipoperoxidation and 4-hydroxynonenal (HNE). We show here for the first time that heterozygote Apc mutation represents a strong selective advantage, via resistance to apoptosis induction (caspase 3 pathway), for colonic cells exposed to a haem-iron induced lipoperoxidation. The fact that HNE treatment of the cells provoked the same effects as the faecal water of rats fed the haem-rich diet suggests that this compound triggers apoptosis in those cells. We propose that this mechanism could be involved in the promotion of colon carcinogenesis by haem in vivo.

Keywords: Colorectal carcinogenesis; haem; Apc gene; faecal water; lipid peroxidation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.