Skip Navigation



Carcinogenesis Advance Access published online on November 17, 2006
Carcinogenesis, doi:10.1093/carcin/bgl217
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
28/5/1104    most recent
bgl217v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cho, H.-J.
Right arrow Articles by Chang, Y.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cho, H.-J.
Right arrow Articles by Chang, Y.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received July 10, 2006
Revised October 9, 2006
Accepted November 2, 2006

CARCINOGENESIS

Ascofuranone Suppresses PMA-mediated matrix metalloproteinase-9 gene activation through the Ras/Raf/MEK/ERK and Ap1-dependent mechanisms

Hyun-Ji Cho 1, Jeong Han Kang 1, Jong-Young Kwak 2, Tae-Sung Lee 3, In-Seon Lee 4, Nam-Gyu Park 5, Hiroo Nakajima 6, Junji Magae 7, and Young-Chae Chang 1 *

1 Department of Pathology, Catholic University of Daegu School of Medicine, Daegu 705-718, Korea
2 Medical Research Center (MRC) for Cancer Molecular Therapy, Dong-A University, Busan, Korea
3 Department of Obstetrics and Gynecology, Catholic University of Daegu School of Medicine, Daegu 705-718, Korea
4 The Center for Traditional Microorganism Resources (TMR), Keimyung University, Daegu 704-701, Korea
5 Department of Biotechnology and Bioengineering and Bioengineering, Pukyong National University, Busan 608-737, Korea
6 Department of Endocrine, Breast Surgery, Kyoto Prefectural University of Medicine, Kyoto, 602-0841, Japan
7 Department of Biotechnology, Institute of Research and Innovation, 1201 Takada, Kashiwa 277-0861, Japan

* To whom correspondence should be addressed.
Young-Chae Chang, E-mail: ycchang{at}cu.ac.kr


  Abstract

The expression of matrix metalloproteinase-9 (MMP-9) has been implicated in the invasion and metastasis of cancer cells. Here we found that an antitumor antibiotic, ascofuranone, inhibits invasion and MMP-9 induction induced by phorbol myristate acetate (PMA) in human cell lines. Ascofuranone also inhibits the protein expression and transcription of MMP-9 induced by tumor necrosis factor-{alpha}. The inhibition of MMP-9 induction was observed in human cancer cell lines as well as primary rat mesangial cells. Furthermore, as evidenced by MMP-9 promoter and electrophoretic mobility shift assays, ascofuranone specifically inhibited MMP-9 gene expression by blocking PMA-stimulated activation of activator protein-1 (AP-1). In addition, ascofuranone suppressed PMA-induced phosphorylation of Ras, Raf, MEK and ERK, upstream factors involved in AP-1activation, while the phosphorylation of p38 and JNK/MAPK was not affected by ascofuranone, suggesting that the primary target of ascofuranone for suppression of the AP-1 inudction is present in upstream of ERK signaling pathway. These results suggest that the suppression of MMP-9 expression, at least in part, contributes to the anti-tumor activity of ascofuranone.

Keywords: Ascofuranone; Matrix Metalloproteinase-9; Activator Protein-1; Osteosarcoma; Tumor invasion.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
P. Montreekachon, P. Chotjumlong, V. Reutrakul, and S. Krisanaprakornkit
Involvement of Cytosolic Phospholipase A2{alpha} in MMP-9 Up-regulation
Journal of Dental Research, November 1, 2009; 88(11): 1031 - 1036.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. K. Park, Y. S. Hwang, K.-K. Park, H.-J. Park, J. Y. Seo, and W.-Y. Chung
Kalopanaxsaponin A inhibits PMA-induced invasion by reducing matrix metalloproteinase-9 via PI3K/Akt- and PKC{delta}-mediated signaling in MCF-7 human breast cancer cells
Carcinogenesis, July 1, 2009; 30(7): 1225 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M.-H. Pan, Y.-S. Chiou, W.-J. Chen, J.-M. Wang, V. Badmaev, and C.-T. Ho
Pterostilbene inhibited tumor invasion via suppressing multiple signal transduction pathways in human hepatocellular carcinoma cells
Carcinogenesis, July 1, 2009; 30(7): 1234 - 1242.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C.-W. Lin, W.-C. Hou, S.-C. Shen, S.-H. Juan, C.-H. Ko, L.-M. Wang, and Y.-C. Chen
Quercetin inhibition of tumor invasion via suppressing PKC{delta}/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma cells
Carcinogenesis, September 1, 2008; 29(9): 1807 - 1815.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S.-O. Lee, Y.-C. Chang, K. Whang, C.-H. Kim, and I.-S. Lee
Role of NAD(P)H:quinone oxidoreductase 1 on tumor necrosis factor-{alpha}-induced migration of human vascular smooth muscle cells
Cardiovasc Res, November 1, 2007; 76(2): 331 - 339.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.