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Carcinogenesis Advance Access published online on January 18, 2007
Carcinogenesis, doi:10.1093/carcin/bgm001
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© The Author 2007. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Pro/antioxidant status and AP-1 transcription factor in murine skin following topical exposure to cumene hydroperoxide

AR Murray1,2, ER Kisin2, C Kommineni2, V Vallyathan2, V Castranova1,2 and AA Shvedova1,2

1 Department Physiology and Pharmacology, West Virginia University, Morgantown, WV, USA
2 Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, WV, USA

All correspondence should be addressed to: Dr. Ashley Murray, Health Effects Laboratory Division, Pathology and Physiology Research Branch, NIOSH, m/s 2015, 1095 Willowdale Road, Morgantown, WV 26505, Tel: (304) 285-5826, FAX: (304) 285-5938, e-mail: zsk1{at}cdc.gov

Organic peroxides, widely used in the chemical and pharmaceutical industries, can act as skin tumor promoters and cause epidermal hyperplasia. They are also known to trigger free radical generation. The present study evaluated the effect of cumene hydroperoxide (Cum-OOH) on the induction of activator protein-1 (AP-1), which is linked to the expression of genes regulating cell proliferation, growth, and transformation. Previously, we reported that topical exposure to Cum-OOH caused formation of free radicals and oxidative stress in the skin of vitamin E deficient mice. The present study used JB6 P+ mouse epidermal cells and AP-1 luciferase reporter transgenic mice to identify whether exposure to Cum-OOH caused activation of AP-1, oxidative stress, depletion of antioxidants, and tumor formation during two-stage carcinogenesis. In vitro studies found that exposure to Cum-OOH reduced the level of glutathione (GSH) in mouse epidermal cells (JB6 P+) and caused the induction of AP-1. Mice primed with dimethyl-benz[a]anthracene (DMBA) were topically exposed to Cum-OOH (82.6 µmol) or the positive control, 12-O-tetradecanoylphorbol-13-acetate (TPA, 17 nmol), twice weekly for 29 weeks. Activation of AP-1 in skin was detected as early as 2 weeks following Cum-OOH or TPA exposure. No AP-1 expression was found 19 weeks post-initiation. Papilloma formation was observed in both the DMBA/TPA and DMBA/Cum-OOH-exposed animals, while skin carcinomas were found only in the DMBA/Cum-OOH-treated mice. A greater accumulation of peroxidative products (TBARS), inflammation, and decreased levels of GSH and total antioxidant reserves were also observed in the skin of DMBA/Cum-OOH exposed mice. These results suggest that Cum-OOH-induced carcinogenesis is accompanied by increased AP-1 activation and changes in antioxidant status.

Key Words: Carcinogens • Hydroperoxides • Skin • Oxidative processes • Occupational hazards


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