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Carcinogenesis Advance Access published online on August 27, 2007
Carcinogenesis, doi:10.1093/carcin/bgm193
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© The Author 2007. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Evaluation of oxidative stress in a group of adolescents exposed to a high-level of aflatoxin B1 - a multi-center and multi-biomarker study

Tao Peng1,2,*, Le-Qun Li1, Min-Hao Peng1, Zhi-Ming Liu1, Tang-Wei Liu1, Ya Guo1, Kai-Yin Xiao1, Zhong Qin1, Xin-Ping Ye1, Xin-Shao Mo1, Lu-Nan Yan3, Bee-Lam Lee4, Han-Ming Shen4, Kazuyoshi Tamae5, LianWen Wang2, Qiao Wang2, Khalid M. Khan2, Kai-bo Wang6, Ren-xiang Liang6, Zong-liang Wei6, Hiroshi Kasai5,*, Choon Nam Ong4,* and Regina M. Santella2,*

1 Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021,Guangxi Province, China
2 Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York NY 10032, USA
3 Department of General Surgery, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan Province, China
4 Department of Community, Occupational and Family Medicine, National University of Singapore, 119260 Singapore
5 Department of Environmental Oncology, Inst. of Industrial Ecological Sciences, University of Occupational & Environmental Health, Kitakyushu 807-8555, Japan
6 Fusui Cancer Institute, Fusui County, 530021, Guangxi Province, China

Correspondence to: Regina M. Santella, Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York NY 10032, USA, rps1{at}columbia.edu.

The association between aflatoxin B1 (AFB1) exposure and oxidative stress was extensively examined in 84 adolescents from an area at high risk for hepatocellular carcinoma in China. Plasma level of AFB1-albumin adducts (AAA) was associated with AFB1 excretion in urine (r=0.394, p<0.001). Urinary AFB1 was also associated with both the urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG) (r≥0.479, p<0.001) and 8-OHdG and hOGG1 levels in peripheral leukocytes (r≥0.308, p≤0.005). Similarly, AAA was significantly associated with both the urinary excretion of 8-OHdG (r≥0.259, p≤0.018) and the 8-OHdG and hOGG1 levels in peripheral leukocytes (r≥0.313, p≤0.004). In addition, urinary 8-OHdG was correlated with both the level of DNA 8-OHdG (r≥0.24, p≤0.05) and the expression of hOGG1 in peripheral leukocytes (r≥0.429, p<0.001). Protein carbonyl content (PCC) level was significantly associated with not only the level of DNA 8-OHdG (r≥0.366, p<0.001) and the urinary 8-OHdG (r≥0.258, p≤0.018), but also the expression of hOGG1 in peripheral leukocytes(r=0.485, p<0.001). A significant but weak association was found between HPLC-ECD and ELISA assays for urinary 8-OHdG (r=0.334, p=0.002), and between HPLC-ECD and flowcytometry assays for 8-OHdG in leucocytes (r=0.395, p<0.001). Significant associations were observed between AAA and PCC and liver function indices (ALT, AST). These findings suggest significant contribution from AFB1 exposure to oxidative stress and subsequent repair among adolescents that may impose substantial risk for hepatocarcinogenesis in adulthood in this region.

* Tao Peng, Hiroshi Kasai, Choon Nam Ong and Regina M. Santella contributed equally to this work.

Received April 25, 2007; revised July 24, 2007; accepted August 7, 2007.


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