Carcinogenesis Advance Access published online on November 4, 2007
Carcinogenesis, doi:10.1093/carcin/bgm233
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Molecular mechanism of black tea polyphenols induced apoptosis in human skin cancer cells: involvement of Bax translocation and mitochondria mediated death cascade
1 Molecular and Human Genetics Division, Indian Institute of Chemical Biology, Kolkata, India
2 Department of Chemistry, Indian Institute of Chemical Biology, Kolkata, India
* Corresponding author Dr. Ashok K. Giri, Molecular and Human Genetics Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata – 700 032, India, Phone: 91-33-2473-3491 / 0492 / 6793, Fax: 91-33-2473-5197, E-mail: akgiri15{at}yahoo.com; akgiri{at}iicb.res.in
Theaflavins (TF) and thearubigins (TR) are the most exclusive polyphenols of black tea. Even though few previous reports showed the anticancer effects of TF through apoptosis, the potential effect of TR has not been appraised. This study investigated the induction of apoptosis in human skin cancer cells after treatment of TR and TF. We report that both TF and TR could exert inhibition of A431 (human epidermoid carcinoma) and A375 (human malignant melanoma) cell proliferation without adversely affecting NHEK (normal human epidermal keratinocytes) cells. Growth inhibition of A375 cells occurred through apoptosis, as evident from cell cycle arrest at G0/G1 phase, increase in early apoptotic cells, externalization of phosphatidyl serine and DNA fragmentation. In our pursuit to dissect the molecular mechanism of TF and TR induced apoptosis in A375 cells, we investigated whether cell death is being mediated by mitochondria. In our system, Bax translocation to mitochondria persuaded depolarization of mitochondrial membrane potential, cytochrome c release in cytosol and induced activation of caspase-9, caspase-3 and PARP (poly (ADP-ribose) polymerase) cleavage. Our intricate investigations on apoptosis also explained that TF and TR augmented Bax/Bcl2 ratio, upregulated the expression of p53 as well as p21 and inhibited phosphorylation of the cell survival protein Akt. Furthermore, TF and TR elicited intracellular ROS (reactive oxygen species) generation in A375 cells. These observations raise speculations that TF as well as TR might exert chemopreventive effect through cell cycle arrest and induction of apoptogenic signals via mitochondrial death cascade in human skin cancer cells.
Received June 20, 2007; revised October 10, 2007; accepted October 18, 2007.