Skip Navigation



Carcinogenesis Advance Access published online on February 24, 2008
Carcinogenesis, doi:10.1093/carcin/bgn052
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
29/8/1587    most recent
bgn052v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Li, X.
Right arrow Articles by Fan, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, X.
Right arrow Articles by Fan, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Adenovirus-delivered CIAPIN1 small interfering RNA inhibits HCC growth in vitro and in vivo

Xiaohua Li, Zhiming Hao, Yanglin Pan, Rui Fan, Haifeng Jin, Shuang Han, Jie Liu, Kaichun Wu and Daiming Fan{ddagger}

State Key Laboratory of Cancer Biology & Institute of Digestive Diseases, Xijing Hospital, the Fourth Military Medical University, 17 Changlexilu, Xi'an, Shaanxi Province, 710032, P.R.China

{ddagger} To whom requests for reprints should be addressed, at State Key Laboratory of Cancer Biology & Institute of Digestive Diseases, Xijing Hospital, the Fourth Military Medical University, 17 Changlexilu, Xi'an, Shaanxi Province, 710032, P.R.China. Tel: 86-29-84773974, Fax: 86-29-82539041, E-mail: fandaimingfdm{at}yahoo.com.cn

Hepatocellular carcinoma (HCC) is an aggressive cancer with a poor prognosis. The specific cellular gene alterations responsible for hepatocarcinogenesis are not well known. CIAPIN1, a recently reported antiapoptotic molecule which plays an essential role in mouse definitive hematopoiesis, is considered a downstream effecter of the receptor tyrosine kinase–Ras signaling pathway. However, the exact function of this gene in tumors is not clear. In this study, we reported that CIAPIN1 is highly expressed in HCC as compared with non-tumor hepatic tissue (p <0.05). We employed adenovirus-mediated RNA interference technique to knock down CIAPIN1 expression in hepatocellular carcinoma cells and observed its effects on hepatocellular carcinoma cell growth in vitro and in vivo. Among the four hepatocellular carcinoma and one normal human liver cell lines we analyzed, CIAPIN1 was highly expressed in hepatocellular carcinoma cells. Knockdown of CIAPIN1 could inhibit HCC cells proliferation by inhibiting the cell cycle S-phase entry. Soft agar colony formation assay indicated that the colony forming ability of SMMC7721 cells decreased by ~70% after adenovirus AdH1-small interfering RNA (siRNA)/CIAPIN1 infection. In vivo experiments showed that adenovirus AdH1-siRNA/CIAPIN1 inhibited the tumorigenicity of SMMC7721 cells and significantly suppressed tumor growth when injected directly into tumors. These results suggest that knockdown of CIAPIN1 by adenovirus-delivered siRNA may be a potential therapeutic strategy for treatment of hepatocellular carcinoma in which CIAPIN1 is overexpressed.

Key Words: CIAPIN1 • adenovirus • tumorigenesis • gene therapy • hepatocellular carcinoma

Received July 11, 2007; revised February 11, 2008; accepted February 12, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.