Skip Navigation



Carcinogenesis Advance Access published online on March 13, 2008
Carcinogenesis, doi:10.1093/carcin/bgn068
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
29/5/913    most recent
bgn068v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ruosaari, S.
Right arrow Articles by Anttila, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruosaari, S.
Right arrow Articles by Anttila, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Aberrations of chromosome 19 in asbestos-associated lung cancer and in asbestos-induced micronuclei of bronchial epithelial cells in vitro

Salla Ruosaari1,2,*, Penny Nymark1,3, Mervi Aavikko1, Eeva Kettunen1, Sakari Knuutila3, Jaakko Hollmén2, Hannu Norppa4 and Sisko Anttila1,3

1 Biological Mechanisms and Prevention of Work-related Diseases, Health and Work Ability, Finnish Institute of Occupational Health, Helsinki, Finland
2 Department of Information and Computer Science, Helsinki University of Technology, Espoo, Finland
3 Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland
4 New Technologies and Risks, Work Environment Development, Finnish Institute of Occupational Health, Helsinki, Finland

* To whom correspondence should be addressed at: Finnish Institute of Occupational Health, Topeliuksenkatu 41 aA, FI-00250 Helsinki, Finland. Tel: +358 30 474 2581; Fax: +358 30 474 2021; Email: salla.ruosaari{at}helsinki.fi

Exposure to asbestos is known to induce lung cancer, and our previous studies have suggested that specific chromosomal regions, such as 19p13, are preferentially aberrant in lung tumours of asbestos-exposed patients. Here, we further examined the association between the 19p region and exposure to asbestos, using array comparative genomic hybridization and fluorescence in situ hybridization (FISH) in lung tumours and FISH characterization of asbestos-induced micronuclei in human bronchial epithelial BEAS 2B cells in vitro. We detected an increased number of 19p losses in the tumours of asbestos-exposed patients in comparison with tumours from non-exposed subjects with similar distribution of tumour histology in both groups (13/33; 39% vs. 3/25; 12%, P=0.04). In BEAS 2B cells, a 48-h exposure to crocidolite asbestos (2.0 µg/cm2) was found to induce centromere-negative micronuclei harbouring chromosomal fragments. Furthermore, an increased frequency of rare micronuclei containing a 19p fragment was observed after the crocidolite treatment in comparison with untreated controls (6/6000 vs. 1/10000, P=0.01). The results suggest that 19p has significance in asbestos-associated carcinogenesis and that asbestos may be capable of inducing specific chromosome aberrations.

Received November 4, 2007; revised February 22, 2008; accepted February 29, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
P. Nymark, E. Kettunen, M. Aavikko, S. Ruosaari, E. Kuosma, E. Vanhala, K. Salmenkivi, R. Pirinen, A. Karjalainen, S. Knuutila, et al.
Molecular Alterations at 9q33.1 and Polyploidy in Asbestos-Related Lung Cancer
Clin. Cancer Res., January 15, 2009; 15(2): 468 - 475.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.