Carcinogenesis Advance Access published online on August 9, 2008
Carcinogenesis, doi:10.1093/carcin/bgn188
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RAGE, carboxylated glycans and S100A8/A9 play essential roles in colitis-associated carcinogenesis
1 La Jolla Institute for Allergy and Immunology, La Jolla, California
2 Department of Pediatrics, University of Münster, Münster, Germany
4 Institute of Experimental Dermatology, University of Münster, Münster, Germany
3 Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, Maryland
5 Tumor Microenvironment Program, Cancer Center, The Burnham Institute for Medical Research, La Jolla, California
6 Department of Medicine, University of Heidelberg, Heidelberg, Germany
7 Department of Pathology, University of California at San Diego, La Jolla, California
* To whom correspondence should be addressed. Tel: 858-795-5256; Fax: 858-713-6281; E-mail: gsrikrishna{at}burnham.org
Patients with inflammatory bowel diseases (IBD) are at increased risk for colorectal cancer, but the molecular mechanisms linking inflammation and cancer are not well defined. We earlier showed that carboxylated N-glycans expressed on RAGE and other glycoproteins mediate colitis through activation of NF-
B. Because NF-B signaling plays a critical role in the molecular pathogenesis of colitis-associated cancer (CAC), we reasoned that carboxylated glycans, RAGE and its ligands might promote CAC. Carboxylated glycans are expressed on a subpopulation of RAGE on colon cancer cells and mediate S100A8/A9 binding to RAGE. Colon tumor cells express binding sites for S100A8/A9 and binding leads to activation of NF-B and tumor cell proliferation. Binding, downstream signaling and tumor cell proliferation are blocked by mAbGB3.1, an anti-carboxylate glycan antibody, and by anti-RAGE. In human colon tumor tissues and in a mouse model of CAC, we found that myeloid progenitors expressing S100A8 and S100A9 infiltrate regions of dysplasia and adenoma. mAbGB3.1 administration markedly reduces chronic inflammation and tumorigenesis in the mouse model of CAC and RAGE-deficient mice are resistant to the onset of CAC. These findings show that RAGE, carboxylated glycans and S100A8/A9 play essential roles in tumor-stromal interactions leading to inflammation associated colon carcinogenesis.
Key Words: RAGE • glycans • S100A8 • S100A9 • colitis • colon cancer
# Current address: Clinical Trials, Capio Diagnostik, Nygaardsvej32, DK2100 Copenhagen, Denmark
Received May 16, 2008; revised July 25, 2008; accepted August 3, 2008.
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