Skip Navigation



Carcinogenesis Advance Access published online on August 13, 2008
Carcinogenesis, doi:10.1093/carcin/bgn192
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrowOA All Versions of this Article:
29/11/2190    most recent
bgn192v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Clarke, J. M.
Right arrow Articles by Cobiac, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clarke, J. M.
Right arrow Articles by Cobiac, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Effects of high amylose maize starch and butyrylated high amylose maize starch on azoxymethane-induced intestinal cancer in rats

Julie M. Clarke1,2,5, David L. Topping1,2, Anthony R. Bird1,2, Graeme P. Young1,3 and Lynne Cobiac4

1 Preventative Health National Research Flagship
2 CSIRO Human Nutrition (a Centre of Food Science Australia), Adelaide, South Australia
3 Flinders Cancer Control Alliance, Flinders University, Bedford Park, South Australia
4 School of Medicine, Flinders University, Bedford Park, South Australia

5 Address correspondence to: PO Box 10041, Adelaide BC 5000 South Australia, Email: julie.clarke{at}csiro.au, Facsimile (ISD): 61 8 8303 8899

Colorectal cancer (CRC) is a major cause of death world wide. Studies suggest that dietary fibre offers protection perhaps by increasing colonic fermentative production of butyrate. This study examined the importance of butyrate by investigating the effects of resistant starch (RS), and butyrylated-RS on azoxymethane (AOM)-induced CRC in rats. Four groups (n = 30/group) of Sprague Dawley rats were fed AIN-93G based-diets containing a standard low-RS maize starch (LAMS), LAMS+3% tributyrin (LAMST), 10% high amylose maize starch (HAMS) and 10% butyrylated HAMS (HAMSB) for 4 weeks. Rats were injected once weekly for 2 weeks with 15mg/kg AOM, maintained on diets for 25 weeks and then sacrificed.

Butyrate concentrations were higher in rats fed HAMSB than other groups in caecal and proximal and distal colonic digesta (P<0.001); levels were similar in HAMS, LAMS and LAMST groups. The proportion of rats developing tumours and the number of tumours per rat were lower in HAMSB than LAMS (P < 0.05). Caecal digesta butyrate pools were negatively correlated with tumour size (P < 0.05). Hepatic portal plasma butyrate concentrations were higher (P < 0.001) in the HAMSB compared with other groups and negatively correlated with tumour number per rat (P < 0.009) and total tumour size for each rat (P=0.05).

HAMSB results in higher luminal butyrate than RS alone or tributyrin. This is associated with reduced tumour incidence, number and size in this rat model of CRC supporting the important protective role of butyrate. Interventional strategies designed to maximise luminal butyrate may be of protective benefit in humans.

Key Words: Colorectal cancer • resistant starch • acylation • rats • butyrate

Received March 19, 2008; revised July 13, 2008; accepted August 1, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Clin. Nutr.Home page
D. L Worthley, R. K Le Leu, V. L Whitehall, M. Conlon, C. Christophersen, D. Belobrajdic, K.-A. Mallitt, Y. Hu, N. Irahara, S. Ogino, et al.
A human, double-blind, placebo-controlled, crossover trial of prebiotic, probiotic, and synbiotic supplementation: effects on luminal, inflammatory, epigenetic, and epithelial biomarkers of colorectal cancer
Am. J. Clinical Nutrition, September 1, 2009; 90(3): 578 - 586.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.