Skip Navigation


Carcinogenesis Advance Access first published online on November 18, 2008
This version published online on December 9, 2008
Carcinogenesis, doi:10.1093/carcin/bgn229
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
30/1/88    most recent
bgn229v2
bgn229v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Newmark, H.
Right arrow Articles by Lipkin, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Newmark, H.
Right arrow Articles by Lipkin, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Western-style diet-induced colonic tumors and their modulation by calcium and vitamin D in C57Bl/6 mice: A preclinical model for human sporadic colon cancer

Harold Newmark1,4, Kan Yang2, Naoto Kurihara2, Kunhua Fan2, Leonard Augenlicht3 and Martin Lipkin2

1 Laboratory for Cancer Research, Rutgers University, Piscataway, NJ
2 Strang Cancer Research Laboratory, New York, NY
3 Albert Einstein College of Medicine, Bronx, NY

4 Correspondence to Dr. Harold L. Newmark, Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 164 Frelinghuysen Road, Piscataway, NJ 08854-8020, Email address: Harold L. Newmark – pnewmark{at}comcast.net

We previously reported that a Western-style diet (NWD) for 18 months, consisting of elevated lipids, and decreased calcium, vitamin D and methyl donor nutrients, induced colonic tumors in normal C57Bl/6 mice [1], suggesting a new mouse model for human sporadic colon cancer. Here, we have extended this study during a longer feeding period of 2 years wherein tumor formation, tumor inhibition by addition of dietary calcium and vitamin D, and their effects on gene expression were determined. We also similarly tested individual supplements of methyl donor (transfer) nutrients (folic acid, choline, methionine and dietary fiber), but these had no significant effect on colonic tumor incidence or multiplicity, while supplementation with combined calcium and Vitamin D produced significant decrease in both colon tumor incidence and multiplicity, during 2 years of feeding. No visible colonic tumors were found at 6 months, very few at 12 months, more at 18 months, significantly at 24 months. In a related study of gene changes of the mouse colonic mucosa at 6 months of feeding taken from this study, long before any tumors were visibly detectable, indicated altered profiles of gene expression linked to later risk of dietary initiation of colon tumor formation. This type of early genetic altered profile, an indication of increased risk of later colonic tumor development, may become a useful tool for prediction of colon tumor risk while the colon grossly still appears histologically and physiologically normal.

Key Words: Western diet • C57Bl/6 mice • Calcium and vitamin D • Methyl donors • Sporadic colon cancer

Received June 6, 2008; revised September 22, 2008; accepted September 24, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Prevention ResearchHome page
K. A. Baltgalvis, F. G. Berger, M. M. O. Pena, J. M. Davis, and J. A. Carson
The Interaction of a High-Fat Diet and Regular Moderate Intensity Exercise on Intestinal Polyp Development in ApcMin/+ Mice
Cancer Prevention Research, July 1, 2009; 2(7): 641 - 649.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.